Dubey, Jitender P.Saville, W. J. A.Sreekumar, C.Shen, S. K.Lindsay, David S.Pena, H. F. J.Vianna, M. C. B.Gennari, S. M.Reed, S. M.2014-06-202014-06-202002-12J. P. Dubey, W. J. Saville, C. Sreekumar, S. K. Shen, D. S. Lindsay, H. F. Pena, M. C. Vianna, S. M. Gennari, and S. M. Reed (2002). "Effects of High Temperature and Disinfectants on the Viability of Sarcocystis neurona Sporocysts," Journal of Parasitology, Vol. 88, No. 6, pp. 1252-1254. doi: http://dx.doi.org/10.1645/0022-3395(2002)088[1252:EOHTAD]2.0.CO;20022-3395http://hdl.handle.net/10919/49041The effect of moist heat and several disinfectants on Sarcocystis neurona sporocysts was investigated. Sporocysts (4 million) were suspended in water and heated to 50, 55, 60, 65, and 70 C for various times and were then bioassayed in interferon gamma gene knockout (KO) mice. Sporocysts heated to 50 C for 60 min and 55 C for 5 min were infective to KO mice, whereas sporocysts heated to 55 C for 15 min and 60 C or more for I min were rendered noninfective to mice. Treatment with bleach (10, 20, and 100%), 2% chlorhexidine, 1% betadine, 5% o-benzyl-p-chlorophenol, 12.56% phenol, 6% benzyl ammonium chloride, and 10% formalin was not effective in killing sporocysts. Treatment with undiluted ammonium hydroxide (29.5% ammonia) for 1 hr killed sporocysts, but treatment with a 10-fold dilution (2.95% ammonia) for 6 hr did not kill sporocysts. These data indicate that heat treatment is the most effective means of killing S. neurona sporocysts in the horse feed or in the environment.application/pdfenIn Copyrightequine protozoal myeloencephalitisopossums didelphis-virginianaexperimental inductioninfectionsraccoonsmiceparasitologyArticle - Refereedhttp://www.bioone.org/doi/abs/10.1645/0022-3395%282002%29088%5B1252%3AEOHTAD%5D2.0.CO%3B2https://doi.org/10.1645/0022-3395(2002)088[1252:eohtad]2.0.co;2