Aryan, AzadehAnderson, Michelle A. E.Myles, Kevin M.Adelman, Zach N.2014-06-162014-06-162013-04Aryan, A.; Anderson, M. A. E.; Myles, K. M.; Adelman, Z. N., "Germline excision of transgenes in Aedes aegypti by homing endonucleases," Scientific Reports 3:1603, (2013). DOI: 10.1038/srep01603.2045-2322http://hdl.handle.net/10919/48929Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.application/pdfenIn Copyrightengineered male mosquitosyellow-fever mosquitohomologousrecombinationdirected evolutionanopheles-gambiaedna breaksgenedrosophilasequencespopulationsmultidisciplinary sciencesArticle - Refereedhttp://www.nature.com/srep/2013/130403/srep01603/full/srep01603.htmlhttps://doi.org/10.1038/srep01603