Mohapatra, Saroj K.Cole, Leah E.Evans, CliveSobral, BrunoBassaganya-Riera, JosepHontecillas, RaquelVogel, Stefanie N.Crasta, Oswald R.2012-08-242012-08-242010-01-18BMC Infectious Diseases. 2010 Jan 18;10(1):10http://hdl.handle.net/10919/18848Background It has been shown previously that administration of Francisella tularensis (Ft) Live Vaccine Strain (LVS) lipopolysaccharide (LPS) protects mice against subsequent challenge with Ft LVS and blunts the pro-inflammatory cytokine response. Methods To further investigate the molecular mechanisms that underlie Ft LVS LPS-mediated protection, we profiled global hepatic gene expression following Ft LVS LPS or saline pre-treatment and subsequent Ft LVS challenge using Affymetrix arrays. Results A large number of genes (> 3,000) were differentially expressed at 48 hours post-infection. The degree of modulation of inflammatory genes by infection was clearly attenuated by pre-treatment with Ft LVS LPS in the surviving mice. However, Ft LVS LPS alone had a subtle effect on the gene expression profile of the uninfected mice. By employing gene set enrichment analysis, we discovered significant up-regulation of the fatty acid metabolism pathway, which is regulated by peroxisome proliferator activated receptors (PPARs). Conclusions We hypothesize that the LPS-induced blunting of pro-inflammatory response in mouse is, in part, mediated by PPARs (α and Ω).application/pdfenCreative Commons Attribution 4.0 InternationalArticle - Refereed2012-08-24Saroj K Mohapatra et al.; licensee BioMed Central Ltd.https://doi.org/10.1186/1471-2334-10-10