Fultz, Stanley Wakefield2014-03-142014-03-141984-12-15etd-07292013-082943http://hdl.handle.net/10919/34249A series of in vitro studies were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) did not differ over the 8 h incubation period. However, the viability of each of the four treatments was lower than that of the unencapsulated control (p<.05 and p<.01; percentage motility and percentage intact acrosomes, respectively), indicating spermatozoal damage occurred during the encapsulation process. Capsule wall thickness and integrity for the .15%, .25%, and .35% concentrations were greater (p<.Ol) than that of the .05% capsules.viii, 65 leavesapplication/pdfIn Copyrightspermatoza viabilityLD5655.V855 1984.F847Dairy cattle -- Artificial inseminationMicroencapsulationSpermatozoa -- ExperimentsThesishttp://scholar.lib.vt.edu/theses/available/etd-07292013-082943/