Yang, Che-HsuehTung, Min-CheLin, Yi-ShengHsu, Chao-YuAkhrymuk, IvanTan, Kok-TongOu, Yen-ChuanLin, Chi-Chien2022-10-262022-10-262022-10-25Yang, C.-H.; Tung, M.-C.; Lin, Y.-S.; Hsu, C.-Y.; Akhrymuk, I.; Tan, K.-T.; Ou, Y.-C.; Lin, C.-C. Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study. Life 2022, 12, 1693.http://hdl.handle.net/10919/112294<b> </b>The study aimed to investigate the therapeutic impact of intravesical instillation of dehydrated human amnion-chorion membrane (HACM) extracts based on the primary pathological feature of interstitial cystitis (IC). We divided 15 female Sprague-Dawley rats into three groups: sham control, IC, and treatment group. IC was induced by 400-µL lipopolysaccharide (1 µg/µL), and it was replaced with normal saline in the sham control group. After IC induction, 300 µL dehydrated HACM extracts (3 mg/kg) were instilled into rats’ urinary bladder weekly for 3 weeks. General histology, inflammatory cytokines, NF-κB, oxidative markers, and western blots results were examined. The urothelial denudation, mast-cell infiltration, and tissues fibrosis were all ameliorated. The elevated TNF-α, IL-1β, IL-6, IL-8, and NF-κB were all down-regulated by dehydrated HACM extracts (<i>p</i> < 0.05). For reactive oxygen species, increased malondialdehyde, decreased superoxide dismutase, and decreased glutathione peroxidase were all reversed (<i>p</i> < 0.05). In apoptosis of IC, elevated Bax and suppressed Bcl-2 were improved (<i>p</i> < 0.05) after instillation. In fibrosis, dysregulated TGFβ/R-Smads/Snail was corrected by the instillation of dehydrated HACM (<i>p</i> < 0.05). In conclusion, dehydrated HACM extracts could be a powerful remedy in treating IC by reconstructing the damaged urothelium, reducing mast-cell infiltration and inflammatory reactions, and ameliorating fibrotic changes.application/pdfenCreative Commons Attribution 4.0 Internationalanimalanti-infective agents/therapeutic useamnion/transplantationcystitisinterstitial/therapychorion transplantationDehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical StudyArticle - Refereed2022-10-26Lifehttps://doi.org/10.3390/life12111693