Elbashir, Salah M.Adnan, Adib M.Bowers, JohnDePaola, AngeloJahncke, MichaelPunchihewage-Don, Anuradha J.Da Silva, Ligia V.Hashem, FawzyParveen, Salina2023-07-282023-07-282023-06-30Elbashir, S.M.; Adnan, A.M.; Bowers, J.; DePaola, A.; Jahncke, M.; Punchihewage-Don, A.J.; Da Silva, L.V.; Hashem, F.; Parveen, S. Antimicrobial Resistance, Virulence Properties and Genetic Diversity of Salmonella Typhimurium Recovered from Domestic and Imported Seafood. Pathogens 2023, 12, 897.http://hdl.handle.net/10919/115935The quantity of seafood imported and produced by domestic aquaculture farming has increased. Recently, it has been reported that multidrug-resistant (MDR) <i>Salmonella</i> Typhimurium may be associated with seafood. However, information is limited to the antimicrobial resistance, virulence properties, and genetic diversity of <i>S. Typhimurium</i> recovered from imported and domestic seafood. This study investigated the antimicrobial resistance, virulence properties, and genetic diversity of <i>S. Typhimurium</i> isolated from domestic and imported catfish, shrimp, and tilapia. A total of 127 isolates were tested for the presence of multidrug-resistance (MDR), virulence genes (<i>inv</i>A, <i>pag</i>C, <i>spv</i>C, <i>spv</i>R), and genetic diversity using the Sensititre micro-broth dilution method, PCR, and pulsed-field gel electrophoresis (PFGE), respectively. All isolates were uniformly susceptible to six (amoxicillin/clavulanic acid, ceftiofur, ceftriaxone, imipenem, nitrofurantoin, and trimethoprim/sulfamethoxazole) of the 17 tested antimicrobials and genetically diverse. Fifty-three percent of the <i>Salmonella</i> isolates were resistant to at least one antimicrobial and 49% were multidrug resistant. Ninety-five percent of the isolates possessed the <i>inv</i>A gene, 67% <i>pag</i>C, and 43% for both <i>spv</i>C, and <i>spv</i>R. The results suggest that <i>S. Typhimurium</i> recovered from seafood is frequently MDR, virulent, and have the ability to cause salmonellosis.application/pdfenCreative Commons Attribution 4.0 InternationalSalmonellaseafoodantibiotic resistanceimporteddomesticvirulence propertiesgenetic diversityArticle - Refereed2023-07-28https://doi.org/10.3390/pathogens12070897