Biase, Fernando H.2022-01-032022-01-032021-122666-1667PMC8536786S2666-1667(21)00601-8 (PII)http://hdl.handle.net/10919/107310Studying individual mammalian oocytes has been extremely valuable for the understanding of the molecular composition of oocytes including RNA storage. Here, a detailed protocol for isolation of oocytes, extraction of total RNA from single oocytes followed by full-length cDNA amplification, and library preparation is presented. The procedure permits the production of cost-effective and high-quality sequencing libraries. This protocol can be adapted for transcriptome analysis of oocytes from other species and be used to generate high-quality data from single embryos. For complete details on the use and execution of this protocol, please refer to Biase and Kimble (2018).Pages 100895application/pdfenCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 InternationalBioinformaticsCell isolationGene ExpressionMolecular BiologyRNAseqSequencingSingle CellProtocol2022-01-03https://doi.org/10.1016/j.xpro.2021.10089524Biase, Fernando [0000-0001-7895-0223]347232122666-1667