Barkefors, IrmeliAidun, Cyrus K.Egertsdotter, Ulrika2017-09-182017-09-182008-01-24Irmeli Barkefors, Cyrus K. Aidun, and E. M. Ulrika Egertsdotter, “Effect of Fluid Shear Stress on Endocytosis of Heparan Sulfate and Low-density Lipoproteins,” Journal of Biomedicine and Biotechnology, vol. 2007, Article ID 65136, 8 pages, 2007. doi:10.1155/2007/65136http://hdl.handle.net/10919/79071Hemodynamic stress is a critical factor in the onset of atherosclerosis such that reduced rates of shear stress occurring at regions of high curvature are more prone to disease. The level of shear stress has direct influence on the thickness and integrity of the glycocalyx layer. Here we show that heparan sulfate, the main component of the glycocalyx layer, forms an intact layer only on cell surfaces subjected to shear, and not under static conditions. Furthermore, receptor-mediated endocytosis of heparan sulfate and low-density liporoteins is not detectable in cells exposed to shear stress. The internalized heparan sulfate and low-density lipoproteins are colocalized as shown by confocal imaging.application/pdfenCreative Commons Attribution 4.0 InternationalArticle - Refereed2017-09-18Copyright © 2007 Irmeli Barkefors et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://doi.org/10.1155/2007/65136