Singhania, RajatTyson, John J.2023-06-272023-06-272023-06-09Singhania, R.; Tyson, J.J. Evolutionary Stability of Small Molecular Regulatory Networks That Exhibit Near-Perfect Adaptation. Biology 2023, 12, 841.http://hdl.handle.net/10919/115535Large-scale protein regulatory networks, such as signal transduction systems, contain small-scale modules (‘motifs’) that carry out specific dynamical functions. Systematic characterization of the properties of small network motifs is therefore of great interest to molecular systems biologists. We simulate a generic model of three-node motifs in search of near-perfect adaptation, the property that a system responds transiently to a change in an environmental signal and then returns near-perfectly to its pre-signal state (even in the continued presence of the signal). Using an evolutionary algorithm, we search the parameter space of these generic motifs for network topologies that score well on a pre-defined measure of near-perfect adaptation. We find many high-scoring parameter sets across a variety of three-node topologies. Of all possibilities, the highest scoring topologies contain incoherent feed-forward loops (IFFLs), and these topologies are evolutionarily stable in the sense that, under ‘macro-mutations’ that alter the topology of a network, the IFFL motif is consistently maintained. Topologies that rely on negative feedback loops with buffering (NFLBs) are also high-scoring; however, they are not evolutionarily stable in the sense that, under macro-mutations, they tend to evolve an IFFL motif and may—or may not—lose the NFLB motif.application/pdfenCreative Commons Attribution 4.0 Internationalperfect adaptationmolecular regulatory networksevolutionary algorithmevolutionary stabilityincoherent feedforward loopsArticle - Refereed2023-06-27https://doi.org/10.3390/biology12060841