Virginia Tech. School of Biomedical Engineering and SciencesUniversity of California, San Diego. Department of Bioengineering and Institute of Engineering in MedicineVirginia Tech. Department of Biomedical Sciences & PathobiologyVirginia Tech. Department of Chemical EngineeringSun, ChenOuyang, MingxingCao, ZhenningMa, SaiAlqublan, HamzehSriranganathan, NammalwarWang, YingxiaoLu, Chang2015-04-202015-04-202014-08-08Sun, C., Ouyang, M., Cao, Z., Ma, S., Alqublan, H., Sriranganathan, N., Wang, Y., Lu, C. (2014). Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding. Chemical Communications, 50(78), 11536-11539. doi: 10.1039/C4CC04730C1359-7345http://hdl.handle.net/10919/51723Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution.application/pdfenCreative Commons Attribution-NonCommercial 3.0 UnportedFluorescence resonance energy transferBiosensorsGenetic encodingArticle - Refereedhttp://pubs.rsc.org/en/content/articlelanding/2014/cc/c4cc04730chttps://doi.org/10.1039/C4CC04730C