Larcom, RavenKietzman, ParryO’Rourke, MeganTracy, Benjamin2025-09-292025-09-292025-09-11Larcom, R.; Kietzman, P.; O’Rourke, M.; Tracy, B. Do Pastures Diversified with Native Wildflowers Benefit Honeybees (Apis mellifera)?Agriculture 2025, 15, 1924.https://hdl.handle.net/10919/137859Tall fescue-dominated pasturelands are widespread in the eastern United States and typically lack substantial plant diversity. Establishing native wildflowers into tall fescue pastures has the potential to benefit bee populations and boost pollinator ecosystem services. In this study, tall fescue pastures at five on-farm sites in Virginia, USA, were planted with wildflowers native to North America and paired with sites with conventional tall fescue pastures. Honeybee apiaries were established at the ten locations, and variables related to hive strength were measured over two years. The main study objectives were to: (1) compare metrics of hive strength between diversified and conventional pastures, (2) determine whether honeybees used native-sown wildflowers as a source of pollen, and (3) explore whether native-sown wildflowers were visited more by honeybees and other pollinators compared with nonnative, unsown forbs. Diversified pastures had many more plant species and blooms compared with conventional pastures, but this had little effect on hive parameters. Pollen DNA metabarcoding revealed that honeybee diets were similar regardless of whether hives were associated with diversified or conventional pastures. Honeybees foraged mostly on plants in the surrounding landscape&mdash;especially white clover (<i>Trifolium repens)</i> and less so on native wildflowers. Native-sown wildflowers received more visits from native pollinators, however. We hypothesize that the native-sown wildflowers had little impact on hive strength metrics because honeybees had access to abundant, white clover blooms and other flowering species in these landscapes. Native wildflowers that bloom in late summer/early autumn after white clover blooms diminish may be of greater value to honeybees in pasture settings.application/pdfenCreative Commons Attribution 4.0 InternationalArticle - Refereed2025-09-26https://doi.org/10.3390/agriculture15181924