2016-08-242016-08-242015-09-15http://hdl.handle.net/10919/72761The present invention provides a novel infectious cDNA clone of porcine reproductive and respiratory syndrome virus (PRRSV), particularly for PRRSV strain VR2385; an improved DNA-launched reverse genetics system for PRRSV; infectious chimeric PRRSV viruses generated through DNA shuffling; modified live-attenuated virus vaccines (MLV) using DNA shuffled chimeric viruses; chimeric viral proteins produced through shuffled chimeric viruses; PRRSV antigens and subunit vaccines based on shuffled chimeric viral proteins; and method of producing broadly protective PRRSV vaccines using DNA shuffling techniques.application/pdfen-USPatenthttp://pimg-fpiw.uspto.gov/fdd/85/321/091/0.pdf137017291/1A61K39/12A61K2039/5254A61K2039/552C12N7/00C12N2770/10034C12N2770/100529132185