Peng, LiangZhang, HuiHao, YuanyuanXu, FeihongYang, JianjunZhang, RuihuaLu, GemingZheng, ZihanCui, MiaoQi, Chen-FengChen, ChunWang, JuanHu, YuanWang, DiPierce, SusanLi, LiwuXiong, Huabao2019-08-282019-08-282016-122352-3964http://hdl.handle.net/10919/93279The regulation of macrophage orientation pathological conditions is important but still incompletely understood. Here, we show that IL-10 and Rag1 double knockout mice spontaneously develop colitis with dominant M1 macrophage phenotype, suggesting that IL-10 regulates macrophage orientation in inflammation. We demonstrate that IL-10 stimulation induced miR-146b expression, and that the expression of miR-146b was impaired in IL-10 deficient macrophages. Our data show that miR-146b targets IRF5, resulting in the regulation of macrophage activation. Furthermore, miR-146b deficient mice developed intestinal inflammation with enhanced M1 macrophage polarization. Finally, miR-146b mimic treatment significantly suppresses M1 macrophage activation and ameliorates colitis development in vivo. Collectively, the results suggest that IL-10 dependent miR-146b plays an important role in the modulation of M1 macrophage orientation. (C) 2016 The Authors. Published by Elsevier B.V.application/pdfenCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 InternationalInterleukin 10miR-146bMacrophageCRISPR/Cas9ColitisArticle - Refereedhttps://doi.org/10.1016/j.ebiom.2016.10.0411427825654