O’Brien, T. J.Bonakdar, MohammadBhonsle, Suyashree P.Neal, Robert E.Aardema, C.H.Robertson, John L.Goldberg, S.N.Davalos, Rafael V.2019-04-092019-04-092018-05-280265-6736http://hdl.handle.net/10919/88874Purpose: This study evaluates the effects of active electrode cooling, via internal fluid circulation, on the irreversible electroporation (IRE) lesion, deployed electric current and temperature changes using a perfused porcine liver model. Materials and methods: A bipolar electrode delivered IRE electric pulses with or without activation of internal cooling to nine porcine mechanically perfused livers. Pulse schemes included a constant voltage, and a preconditioned delivery combined with an arc-mitigation algorithm. After treatment, organs were dissected, and treatment zones were stained using triphenyl-tetrazolium chloride (TTC) to demonstrate viability. Results: Thirty-nine treatments were performed with an internally cooled applicator and 21 with a non-cooled applicator. For the constant voltage scenario, the average final electrical current measured was 26.37 and 29.20 A for the cooled and uncooled electrodes respectively (p≤.001). The average final temperature measured was 33.01 and 42.43 °C for the cooled and uncooled electrodes respectively (p≤.0001). The average measured ablations (fixed lesion) were 3.88-by-2.08 cm and 3.86-by-2.12 cm for the cooled and uncooled electrode respectively (p≤.2495, p≤.7507). Similarly, the preconditioned/arc-mitigation scenario yielded an average final electrical current measurement of a 41.07 and 47.20 A for the cooled and uncooled electrodes respectively (p≤.0001). The average final temperature measured was 34.93 and 44.90 °C for the cooled and uncooled electrodes respectively (p≤.0001). The average measured ablations (fixed lesion) were 3.67-by-2.27 cm and 3.58-by-2.09 cm for the cooled and uncooled applicators ((p≤.7906; p≤.5595)). Conclusions: The internally-cooled bipolar applicator offers advantages that could improve clinical outcomes. Thermally mitigating internal perfusion technology reduced tissue temperatures and electric current while maintaining similar lesion sizes.application/pdfen-USCreative Commons Attribution 4.0 Internationalarc mitigationcurrentIrreversible electroporationperfused organ modelTemperaturethermal damagethermal mitigationArticle - Refereedhttps://doi.org/10.1080/02656736.2018.147389335129806513