Virginia TechSolomon, J. E.Paul, Mark R.2014-02-262014-02-262006-03-01Solomon, JE; Paul, MR. "The kinetics of analyte capture on nanoscale sensors," Biophysical Journal 90(5), 1842-1852 (2006); doi: 10.1529/biophysj.105.0678350006-3495http://hdl.handle.net/10919/25779This article presents a number of kinetic analyses related to binding processes relevant to capture of target analyte species in nanoscale cantilever-type devices designed to detect small concentrations of biomolecules. The overall analyte capture efficiency is a crucial measure of the ultimate sensitivity of such devices, and a detailed kinetic analysis tells us how rapidly such measurements may be made. We have analyzed the capture kinetics under a variety of conditions, including the possibility of so-called surface-enhanced ligand capture. One of the modalities studied requires ligand capture through a cross-linking mechanism, and it was found that this mode may provide a robust and sensitive approach to biomolecular detection. For the two modalities studied, we find that detection of specific biomolecules down to concentration levels of 1 nM or less appear to be quite feasible for the device configurations studied.application/pdfen-USIn CopyrightCoupled chemical-reactionsDynamic force microscopyReceptorsCross-linkingCell-surfaceFunctional-groupsDiffusionArticle - Refereedhttp://www.sciencedirect.com/science/article/pii/S0006349506723712https://doi.org/10.1529/biophysj.105.067835