He, SijiaWaheed, Abdul A.Hetrick, BrianDabbagh, DeemahAkhrymuk, Ivan V.Kehn-Hall, KyleneFreed, Eric O.Wu, Yuntao2021-01-082021-01-082020-12-30He, S.; Waheed, A.A.; Hetrick, B.; Dabbagh, D.; Akhrymuk, I.V.; Kehn-Hall, K.; Freed, E.O.; Wu, Y. PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity. Viruses 2020, 13, 46.http://hdl.handle.net/10919/101800P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1⁺ cellsapplication/pdfenCreative Commons Attribution 4.0 Internationalcoronaviruscellular antiviral factorpseudovirionArticle - Refereed2021-01-08https://doi.org/10.3390/v13010046