Majeed, Haniyyah JaRae2022-04-162022-04-162020-10-22vt_gsexam:27741http://hdl.handle.net/10919/109682Wastewater treatment plants (WWTPs) receive a confluence of sewage containing antibiotics, antibiotic resistant bacteria, antibiotic resistance genes (ARGs), and pathogens, thus serving as key point of interest for the surveillance of antibiotic resistance (AR) dissemination. This thesis advances knowledge about the fate of AR indicators throughout treatment and reuse. The field study informs approaches for monitoring AR at a WWTP by characterizing the resistome (i.e., full profile of ARGs) and microbiome across eight sampling events via metagenomic sequencing, complemented by antibiotic data. The WWTP significantly reduced the total load of ARGs and antibiotics, although correlations between ARGs and antibiotics were generally weak. Quantitative polymerase chain reaction was applied to validate the quantitative capacity of metagenomics, whereby we found strong correlations. The influent and effluent to the WWTP were remarkably stable with time, providing further insight into the sampling frequency necessary for adequate surveillance. The laboratory study examined the effects of commonly applied disinfection processes (chlorination, chloramination, and ultraviolet irradiation [UV]) on the inactivation of antibiotic resistant pathogens and corresponding susceptible pathogens in recycled and potable water. Further, we evaluated their regrowth following disinfection by simulating distribution. Acinetobacter baumannii, an environmental opportunistic pathogen, regrew especially well following UV disinfection, although not when a disinfectant residual was present. Enterococcus faecium, a fecal pathogen, did not regrow following any disinfection process. There were no significant differences between water types. The findings of this study emphasize a need to move beyond the framework of assessing treatment efficacy based on the attenuation of fecal pathogens.ETDIn Copyrightantibiotic resistancewastewater treatmentwastewater reclamationThesis