Bennett, Lisa Tracy2014-03-142014-03-141989-12-15etd-02062013-040043http://hdl.handle.net/10919/40938Nitrogenase-catalyzed nitrogen fixation is a biochemically and genetically complex process requiring the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from <i>Azotobacter vinelandii</i> were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in <i>A vinelandii</i> flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be constitutively expressed but under nitrogen fixing conditions nifF gene expression is stimulated.ix, 142 leavesBTDapplication/pdfenIn CopyrightLD5655.V855 1989.B466Klebsiella pneumoniaeMutagenesisThesishttp://scholar.lib.vt.edu/theses/available/etd-02062013-040043/