Barbosa, Anne C.Xu, ZhengyaoKarari, KazhalWilliams, WendiHauf, SilkeBrown, William R. A.2022-09-022022-09-022022-01-110305-1048http://hdl.handle.net/10919/111692We have used chromosome engineering to replace native centromeric DNA with different test sequences at native centromeres in two different strains of the fission yeast Schizosaccharomyces pombe and have discovered that A + T rich DNA, whether synthetic or of bacterial origin, will function as a centromere in this species. Using genome size as a surrogate for the inverse of effective population size (N-e) we also show that the relative A + T content of centromeric DNA scales with N-e across 43 animal, fungal and yeast (Opisthokonta) species. This suggests that in most of these species the A + T content of the centromeric DNA is determined by a balance between selection and mutation. Combining the experimental results and the evolutionary analyses allows us to conclude that A + T rich DNA of almost any sequence will function as a centromere in most Opisthokonta species. The fact that many G/C to A/T substitutions are unlikely to be selected against may contribute to the rapid evolution of centromeric DNA. We also show that a neo-centromere sequence is not simply a weak version of native centromeric DNA and suggest that neo-centromeres require factors either for their propagation or establishment in addition to those required by native centromeres.application/pdfenCreative Commons Attribution 4.0 Internationalcenp-aartificial chromosomesheterochromatinevolutionratescomplementationneocentromeresconstructionrequirementassociationArticle - Refereedhttps://doi.org/10.1093/nar/gkab1219501349283841362-4962