Artemov, Gleb N.Stegniy, Vladimir N.Sharakhova, Maria V.Sharakhov, Igor V.2018-09-212018-09-212018-09-17Artemov, G.N.; Stegniy, V.N.; Sharakhova, M.V.; Sharakhov, I.V. The Development of Cytogenetic Maps for Malaria Mosquitoes. Insects 2018, 9, 121.http://hdl.handle.net/10919/85070Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a <i>Plasmodium</i> transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for <i>Anopheles beklemishevi.</i>application/pdfenCreative Commons Attribution 4.0 Internationalcytogenetic mapchromosome mapmalaria mosquitoesAnophelespolytene chromosomesAnopheles beklemishevichromosome map developmentphotomaphigh-pressure techniquechromosome straighteningArticle - Refereed2018-09-21https://doi.org/10.3390/insects9030121