Ranjan, AshishPothayee, NikornSeleem, Mohamed N.Tyler, Ronald D.Brenseke, BonnieSriranganathan, NammalwarRiffle, Judy S.Kasimanickam, Ramanathan K.2020-09-212020-09-212009-01-011178-2013http://hdl.handle.net/10919/100047Pluronic based core-shell nanostructures encapsulating gentamicin were designed in this study. Block copolymers of (PAA(+/-)Na-b-(PEO-b-PPO-b-PEO)-b-PAA(+/-)Na) were blended with PAA(-) Na(+) and complexed with the polycationic antibiotic gentamicin to form nanostructures. Synthesized nanostructures had a hydrodynamic diameter of 210 nm, zeta potentials of -0.7 (+/-0.2), and incorporated approximately 20% by weight of gentamicin. Nanostructures upon co-incubation with J774A.1 macrophage cells showed no adverse toxicity in vitro. Nanostructures administered in vivo either at multiple dosage of 5 microg g(-1) or single dosage of 15 microg g(-1) in AJ-646 mice infected with Salmonella resulted in significant reduction of viable bacteria in the liver and spleen. Histopathological evaluation for concentration-dependent toxicity at a dosage of 15 microg g(-1) revealed mineralized deposits in 50% kidney tissues of free gentamicin-treated mice which in contrast was absent in nanostructure-treated mice. Thus, encapsulation of gentamicin in nanostructures may reduce toxicity and improve in vivo bacterial clearance.Pages 289-2979 page(s)Print-Electronicapplication/pdfenCreative Commons Attribution-NonCommercial 4.0 InternationalLife Sciences & BiomedicineNanoscience & NanotechnologyPharmacology & PharmacyScience & Technology - Other Topicsgentamicincore-shell nanostructuresSalmonellaPLURONIC(R) BLOCK-COPOLYMERSINDUCED NEPHROTOXICITYDRUG-DELIVERYLIPOSOMESNANOPARTICLESNANOCARRIERSRELEASE0601 Biochemistry and Cell Biology1007 Nanotechnology1115 Pharmacology and Pharmaceutical SciencesNanoscience & NanotechnologyAnimalsMiceSalmonellaSalmonella InfectionsGentamicinsCoated Materials, BiocompatibleDrug CarriersAnti-Bacterial AgentsCrystallizationMaterials TestingDrug CompoundingDiffusionDose-Response Relationship, DrugParticle SizeSurface PropertiesNanostructuresNanomedicineArticle - Refereed2020-09-21https://doi.org/10.2147/ijn.s71374Sriranganathan, Nammalwar [0000-0002-9232-2888]Seleem, Mohamed [0000-0003-0939-0458]20054433 (pubmed)1178-2013