Virginia TechLin, Y. C.Cheng, C. J.Poon, Ting-Chung2014-03-142014-03-142011-03-01Yu-Chih Lin, Chau-Jern Cheng, and Ting-Chung Poon, "Optical sectioning with a low-coherence phase-shifting digital holographic microscope," Appl. Opt. 50, B25-B30 (2011); doi: 10.1364/ao.50.000b251559-128Xhttp://hdl.handle.net/10919/25898The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3 mu m and axial resolution of 10 mu m through a thickness of 300 mu m onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment. (C) 2010 Optical Society of Americaapplication/pdfenIn CopyrightScanning holography3-dimensional microscopyInterference holographyImage-reconstructionTomographyArticle - Refereedhttp://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-7-B25https://doi.org/10.1364/ao.50.000b25