School of Animal Sciences

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https://hdl.handle.net/10919/111086
The School of Animal Sciences merged Dairy Science and Animal and Poultry Science in 2022.

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Browsing School of Animal Sciences by Author "Afrin, Fahmida"

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    Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection
    Diaz, Joyce Anne R.; Kim, Woo H.; Fernandez, Cherry P.; Jeong, Jipseol; Afrin, Fahmida; Lillehoj, Hyun S.; Kim, Suk; Kim, Sungwon; Dalloul, Rami A.; Min, Wongi (2016-08)
    Interleukin (IL)-17D is a proinfiammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. dull-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinfiammatory cytokines such as IL-6, IL-8, and IL-1 beta in duck embryo fibroblast cells. Very low levels of dull-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The dull-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species.