Browsing by Author "Azimirad, Masoumeh"

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    Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
    Ghavami, Shaghayegh Baradaran; Yadegar, Abbas; Aghdaei, Hamid Asadzadeh; Sorrentino, Dario; Farmani, Maryam; Mir, Adil Shamim; Azimirad, Masoumeh; Balaii, Hedieh; Shahrokh, Shabnam; Zali, Mohammad Reza (2020-09)
    In inflammatory bowel diseases (IBD), the therapeutic benefit and mucosal healing from specific probiotics may relate to the modulation of dendritic cells (DCs). Herein, we assessed the immunomodulatory effects of four probiotic strains includingLactobacillus salivarius,Bifidobacterium bifidum,Bacillus coagulansandBacillus subtilisnatto on the expression of co-stimulatory molecules, cytokine production and gene expression of signal-transducing receptors in DCs from IBD patients. Human monocyte-derived DCs from IBD patients and healthy controls were exposed to four probiotic strains. The expression of co-stimulatory molecules was assessed and supernatants were analyzed for anti-inflammatory cytokines. The gene expression of toll-like receptors (TLRs), IL-12p40 and integrin alpha v beta 8 were also analyzed. CD80 and CD86 were induced by most probiotic strains in ulcerative colitis (UC) patients whereas onlyB. bifiduminduced CD80 and CD86 expression in Crohn's disease (CD) patients. IL-10 and TGF-beta production was increased in a dose-independent manner while TLR expression was decreased by all probiotic bacteria exceptB. bifidumin DCs from UC patients. TLR-4 and TLR-9 expression was significantly downregulated while integrin ss8 was significantly increased in the DCs from CD patients. IL-12p40 expression was only significantly downregulated in DCs from CD patients. Our findings point to the general beneficial effects of probiotics in DC immunomodulation and indicate that probiotic bacteria favorably modulate the expression of co-stimulatory molecules, proinflammatory cytokines and TLRs in DCs from IBD patients.