Browsing by Author "Bills, Gerald F."
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- Ecological and taxonomic studies of the Russulaceae and other ectomycorrhizal Basidiomycetes in the high-elevation forests of the southern AppalachiansBills, Gerald F. (Virginia Polytechnic Institute and State University, 1985)Temperate and boreal fungal floras indicate that species of the Russulaceae (the genera Russula andLactarius) are among the dominant ectomycorrhizal fungi in forest communities. The contribution of the Russulaceae to the communities of ectomycorrhizal Basidiomycetes fruiting in red spruce and adjacent northern hardwood forests in West Virginia was evaluated and compared with other ectomycorrhizal Basidiomycetes occupying the same habitats. The Russulaceae exhibited the greatest species diversity of any family of ectomycorrhizal fungi fruiting in the stands studied (44% of the species in spruce, 39% of the species in hardwoods). Species of Lactarius and Russula were among the most productive in both forests. Species diversity, productivity, and fruiting phenology of all ectomycorrhizal Basidiomycetes were compared between red spruce and northern hardwood stands for a 3-year period. Sporocarp numbers and sporocarp frequency in 384 four m² quadrats in each forest type was used to estimate productivity. Species richness was greater in hardwoods (36 species) than in spruce (27 species). Nine species were common to both forests. Most productivity was concentrated in a few species, while most species were rare. Species-area curves were constructed for both forests. Fungal species and tree species composition in both forests were compared by principal component analysis. Fungi in spruce forests were more productive than in hardwood forests. Productivity was highly variable among the three seasons studied because of climatic variability. Sporocarp abundance and frequency were positively correlated with basal area and density of mycorrhizal trees and were negatively correlated with fern cover in hardwood forests. Fruiting seasons extended from early July to late September or early October. Numbers of species fruiting from the same four m² quadrats ranged from 0 to 7 in spruce forests and 0 to 5 in hardwood forests. Spatial patterns of sporocarps of major species were characterized by the variance-to-mean ratio, mean crowding, patchiness, and spatial autocorrelation and were found to exhibit highly aggregated, contagious patterns. Interspecific associations between pairs of major species were measured by 2 x 2 contingency tables and Cole's index of association. A taxonomie and geographic survey of Russula and Lactarius in both the quantitative study areas and in similar habitats in the Southern Appalachians was presented.
- Phylogenetic and Chemotaxonomic Studies Confirm the Affinities of Stromatoneurospora phoenix to the Coprophilous XylariaceaeBecker, Kevin; Wongkanoun, Sarunyou; Wessel, Anna-Charleen; Bills, Gerald F.; Stadler, Marc; Luangsa-ard, J. Jennifer (MDPI, 2020-08-23)The genus Stromatoneurospora was erected in 1973 by Jong and Davis to accommodate the pyrophilic pyrenomycete Sphaeria phoenix and has traditionally been placed in the family Xylariaceae based on morphological features. However, no living culture of this genus has so far been available in the public domain. Molecular data were restricted to an internal transcribed spacer (ITS) sequence that only confirmed the familial position, and was generated from a strain that is not deposited in a public culture collection. We have recently collected fresh material and were able to culture this fungus from Thailand. The secondary metabolites of this strains were analysed after fermentation in multiple media. The the prominent components of these fermentation were purified, using preparative chromatography. Aside from two new eremophilane sesquiterpenoids named phoenixilanes A–B (1–2), four other components that are known from species of the xylariaceous genera Xylaria and Poronia were identified by spectral methods (nuclear magnetic resonance spectroscopy and high resolution mass spectrometry). Notably, (−)-(R)-6-hydroxy-3-methyl-4-dihydroisocoumarin-5-carboxylic acid (6) has not been reported as a natural product before. Moreover, DNA sequences of Stromatoneurospora phoenix clustered with members of the genera Poronia and Podosordaria in a multi-locus molecular phylogeny. These results confirmed that the genus belongs to the same evolutionary lineage as the coprophilic Xylariaceae. The results also suggest that this lineage has evolved independently from the plant-inhabiting saprotrophs and endophytes that are closely related to the genus Xylaria. These findings are discussed in relation to some theories about the endophytic vs. the pyrophilic/coprophilic fungal life style.
- Sphaerostilbellins, New Antimicrobial Aminolipopeptide Peptaibiotics from Sphaerostilbella toxicaPerlatti, Bruno; Nichols, Connie B.; Alspaugh, J. Andrew; Gloer, James B.; Bills, Gerald F. (MDPI, 2020-09-26)Sphaerostilbella toxica is a mycoparasitic fungus that can be found parasitizing wood-decay basidiomycetes in the southern USA. Organic solvent extracts of fermented strains of S. toxica exhibited potent antimicrobial activity, including potent growth inhibition of human pathogenic yeasts Candida albicans and Cryptococcus neoformans, the respiratory pathogenic fungus Aspergillus fumigatus, and the Gram-positive bacterium Staphylococcus aureus. Bioassay-guided separations led to the purification and structure elucidation of new peptaibiotics designated as sphaerostilbellins A and B. Their structures were established mainly by analysis of NMR and HRMS data, verification of amino acid composition by Marfey’s method, and by comparison with published data of known compounds. They incorporate intriguing structural features, including an N-terminal 2-methyl-3-oxo-tetradecanoyl (MOTDA) residue and a C-terminal putrescine residue. The minimal inhibitory concentrations for sphaerostilbellins A and B were measured as 2 μM each for C. neoformans, 1 μM each for A. fumigatus, and 4 and 2 μM, respectively, for C. albicans. Murine macrophage cells were unaffected at these concentrations.