Browsing by Author "Cheever, B. M."
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- The relative importance of exogenous and substrate-derived nitrogen for microbial growth during leaf decompositionCheever, B. M.; Webster, Jackson R.; Bilger, E. E.; Thomas, S. A. (Ecological Society of America, 2013-07)Heterotrophic microbes colonizing detritus obtain nitrogen (N) for growth by assimilating N from their substrate or immobilizing exogenous inorganic N. Microbial use of these two pools has different implications for N cycling and organic matter decomposition in the face of the global increase in biologically available N. We used sugar maple leaves labeled with N-15 to differentiate between microbial N that had been assimilated from the leaf substrate (enriched with N-15) or immobilized from the water (natural abundance N-15:N-14) in five Appalachian streams ranging in ambient NO3-N concentrations from about 5 to 900 g NO3-N/L. Ambient NO3- concentration increased sugar maple decomposition rate but did not influence the proportion of microbial N derived from substrate or exogenous pools. Instead, these proportions were strongly influenced by the percentage of detrital ash-free dry mass (AFDM) remaining. Substrate-derived N made up a large proportion of the microbial N after the first 24 h in all streams. Detrital and microbial isotopic N-15 signatures approached that of the water as decomposition progressed in all streams, suggesting that exogenous N may be the predominant source of N for meeting microbial requirements even when exogenous N concentrations are low. Our results support predictions of more rapid decomposition of organic matter in response to increased N availability and highlight the tight coupling of processes driving microbial N cycling and organic matter decomposition.