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Browsing by Author "Doria, Sofia"

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    Chromosome mis-segregation and cytokinesis failure in trisomic human cells
    Nicholson, Joshua M.; Macedo, Joana C.; Mattingly, Aaron J.; Wangsa, Darawalee; Camps, Jordi; Lima, Vera; Gomes, Ana M.; Doria, Sofia; Ried, Thomas; Logarinho, Elsa; Cimini, Daniela (eLife, 2015-05-05)
    Cancer cells display aneuploid karyotypes and typically mis-segregate chromosomes at high rates, a phenotype referred to as chromosomal instability (CIN). To test the effects of aneuploidy on chromosome segregation and other mitotic phenotypes we used the colorectal cancer cell line DLD1 (2n = 46) and two variants with trisomy 7 or 13 (DLD1+7 and DLD1+13), as well as euploid and trisomy 13 amniocytes (AF and AF+13). We found that trisomic cells displayed higher rates of chromosome mis-segregation compared to their euploid counterparts. Furthermore, cells with trisomy 13 displayed a distinctive cytokinesis failure phenotype. We showed that up-regulation of SPG20 expression, brought about by trisomy 13 in DLD1+13 and AF+13 cells, is sufficient for the cytokinesis failure phenotype. Overall, our study shows that aneuploidy can induce chromosome mis-segregation. Moreover, we identified a trisomy 13-specific mitotic phenotype that is driven by up-regulation of a gene encoded on the aneuploid chromosome.