Browsing by Author "Feuerbacher, Erica N."

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    Can Dogs Learn Concepts the Same Way We Do? Concept Formation in a German Shepherd
    Feuerbacher, Erica N.; Rosales-Ruiz, Jesus (International Society For Comparative Psychology, 2017-01-01)
    Growing evidence shows that dogs can complete complex behavioral tasks, such as learning labels for hundreds of objects, readily learning the name of a novel object, and responding differentially to objects by category (e.g., “toy,” “ball,” “Frisbee”). We expand here on the evidence for complex behavioral abilities in dogs by demonstrating that they are capable of concept formation by strict criteria. A German shepherd responded differentially to two sets of objects (“toys” and “non-toys”) in Experiment 1. Additionally, the dog’s differential responding in Experiment 1 occurred from the first trial, indicating that he entered the experiment with this stimulus class already differentiated from his day-to-day exposure to contingencies. In Experiment 2 we used a common response (tug-of-war) with three objects that were not retrieved in Experiment 1 to attempt to add these objects to the stimulus class. After repeated sessions of tug-of-war, the dog began retrieving all three objects in the retrieval test, although the rates of retrieval varied between objects. Finally, in Experiment 3, we conducted a transfer of function test in which the dog emitted a new response to untrained exemplars suggesting that his differential responding in Experiment 1 was indicative of a concept by the strictest criteria. Additionally, the reliably emitted the new response in the transfer test to one of the three new objects from Experiment 2, suggesting this object had been reliably added to the conceptual class.
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    Canine Nosework as an Intervention for Behavior Change in Shelter Dogs
    Semione, Nisa (Virginia Tech, 2024-05-08)
    Millions of dogs enter the shelter annually in the United States. Dogs living in shelters experience a variety of stressors that can contribute to impaired welfare. Shelter enrichment programs are one way to mitigate these stressors and improve the welfare of kenneled dogs. This study compared the effects of nosework activities and positive human social contact via petting on the behavior of kenneled shelter dogs in response to an unknown person approaching the kennel. The present study found that dogs exhibited three behaviors most often in response to an unknown person approaching the kennel, 1) barking, 2) standing, and 3) jumping on the kennel. There were no effects found in either intervention on the proportion of time spent jumping on the kennel. Dogs exhibited increased standing behavior in their kennels after both interventions. There were no significant effects of the petting intervention on the proportion of time spent barking. The nosework intervention demonstrated an increase in the proportion of time spent barking the day-after the intervention, indicating a possible frustration response.
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    Case study of perceived positive youth development inputs and outcomes in 4-H dog programs as identified by 4-H members, parents, leaders and agents
    McGraw, Carolyn Marie (Virginia Tech, 2025-01-06)
    There is a recognized need for additional research that produces empirical data on positive youth development (PYD) frameworks, as well as PYD impacts and roles amongst human-animal interactions (HAI) and youth. This study used inputs and outcomes from the 4-H Formula for Success to identify and describe components of the 4-H dog program within one state as case studies. Inputs included skill-building, meaningful leadership, and long-term relationships with caring adults. Outcomes included the 5 C's: caring, character, confidence, connection and competence. Three counties (cases) were identified, and participants were interviewed about their 4-H dog program experiences. 4-H youth, parents, volunteers and agents were included. A total of 19 interviews were transcribed and coded for themes that fit within the 4-H Formula for Success within the context of each case. Findings indicate a strong presence of inputs that are specific to dog programming, as well as some outcomes and emergent themes, including accessibility, motivation to join and COVID implications. Dog industry professionals greatly contributed to the skill-building and meaningful leadership opportunities for 4-H youth. Connection and Competence were the most frequently coded outcomes across the cases. Participants described the 4-H dog program as accessible due to cost, small size and simple housing requirements, compared to livestock or equine projects. A shared interest in dogs was the primary motivation to join 4-H dog clubs and led to strong connections or bonds between members. Limitations of this study are related to a low participant pool in one case, and recall accuracy and cognitive ability of 4-H youth to recognize and name their perceived outcomes. Recommendations for practice are included for 4-H agents and 4-H volunteers to start, promote or strengthen 4-H dog programs in any location by being intentional when recruiting 4-H volunteers, and planning educational programming. This program can be promoted as more accessible compared to large animal science 4-H programs such as livestock or equine based on data provided by participants in all three cases within diverse contexts. There are several recommendations for further research beyond this study including a longitudinal continuation of this model, replicating this study in other states to examine other 4-H dog programs, or replicating this study for other 4-H program areas.
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    Chicken or fish? Do environmental complexity and stocking density impact affective states of broiler chickens and rainbow trout?
    Anderson, Mallory G. (Virginia Tech, 2021-09-30)
    In commercial settings, broiler chickens and rainbow trout are housed in barren environments under high stocking densities, due to an emphasis on production efficiency. These monotonous housing conditions do not provide broilers or trout with the ability to perform functional, highly-motivated behaviors and increase their susceptibility to excessive anxiety and fear, resulting in negative affective states and poor animal welfare. Affective state (or emotional state) is a cumulative product of short-term life experiences, ranging from positive to negative. Because affective states are largely influenced by environmental condition, determining animal affective state can provide useful information on how to improve housing conditions in order to ensure positive experiences and good animal welfare. Cognitive processes are closely associated with affective state; a "cognitive bias" occurs when affective state influences aspects of cognition, such as judgement and attention. Animals in positive affective states make optimistically-biased decisions during ambiguous situations, judging the situation as if it will produce a positive outcome, and show less bias towards a perceived threat, responding in a less anxious and calm manner. Animals in negative affective states make pessimistically-biased decisions during ambiguous situations, judging the situation as if it will result in a negative outcome. Additionally, animals in negative affective states will bias their attention towards a perceived threat rather than alternative stimuli, responding in an anxious manner. Therefore, judgement and attention bias tests can be used to determine animal affective states. In Chapter 3, a judgement bias test was used to determine affective state of broiler chickens housed in either complex (perches, dust bath, pecking stones, and rotating enrichment objects) or barren (no enrichment) environments under either high or low stocking densities. Broilers housed in complex environments responded more optimistically during the judgement bias test than broilers from barren environments, indicating the former were in a positive affective state. Stocking density did not impact their responses in the judgement bias test, indicating that affective states were not impacted by that treatment. In Chapter 4, an attention bias test was used to determine level of anxiety and a tonic immobility test was used to determine fear in order to investigate affective state of broilers housed in the same conditions as described for Chapter 3. Broilers housed in complex environments were less anxious during the attention bias test than broilers from barren environments, indicating environmental complexity reduced anxiety in broilers. Stocking density did not impact anxiety. Broilers from high stocking density environments had shorter tonic immobility durations than broilers from low stocking density environments, suggesting the former were less fearful. Environmental complexity did not impact fearfulness. In Chapter 5, a judgement bias test was used to determine affective state of rainbow trout housed in either complex (shelter structure and artificial plants) or barren (no enrichment) tanks under either low or high stocking densities. Trout housed in high stocking density tanks responded optimistically during the judgement bias test, indicating they were in a more positive affective state compared to trout housed in low stocking density tanks. Environmental complexity did not impact their responses in the judgement bias test, indicating no effect of enrichments on affective states was found. These results indicate a beneficial relationship of a complex environment on broiler chicken affective state, observed through an optimistic judgement bias and reduced attention bias (anxiety) towards a perceived threat. Thus, providing a complex housing environment for broilers can improve their welfare and result in a positive affective state. Rainbow trout reared at the tested high density resulted in a positive affective state, although complexity did not benefit their welfare. Our results contribute much needed information on stocking densities to ensure fish welfare. Overall, environmental complexity, not stocking density, had a positive impact on broiler chicken affective states. Rainbow trout affective states were positively impacted by stocking density, but not environmental complexity.
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    Citizen Science: Training Pet Dogs to Detect the Spotted Lanternfly
    Decker, Hannah (Virginia Tech, 2021-09-16)
    Dogs have been used alongside humans as detection tools for centuries. There have been a multitude of studies published that demonstrate the accuracy and utility of detection dogs, more specifically conservation scent detection dogs. With ubiquitous agricultural threats in the United States, there is a need for a tool to help decrease the threat level. Pet dogs could be the answer. There are millions of pet dogs in the United States and with the success of the dog sport nose work there is the potential to use pet dogs as detection tools. In this proof-of-concept study, six pet dogs were trained to detect the spotted lanternfly. The dogs completed a training phase and five tests. The mean sensitivity of the six dogs, for the five tests, was 79.75%. The mean PPP of the six dogs, for all five tests, was 66.79%. The results suggest that these six dogs could be beneficial detection tools for the Spotted Lanternfly. Based on the findings in this study, pet dogs could be invaluable in the field of conservation scent detection.
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    Comparing the Differential Effects of Neighborhood and Nature Walks on Behavior and Urinary Cortisol Levels of Dogs
    Cupp, Glenna N.; Feuerbacher, Erica N.; Gunter, Lisa M.; Hekman, Jessica P. (Virginia Tech, 2023-12-07)
    Dog training practitioners suggest that walks in nature provide more welfare enhancing benefits than the neighborhood walks that most dogs experience daily. While the benefits of nature walks are a well-studied phenomenon in humans, to date there has been little investigation of this topic in companion dogs using objective measures. This study compared the effects of thirty-minute walks in nature to walks in the dogs’ home neighborhoods. Fifteen dogs took part in a within- subjects design that measured physiology and behavior to examine the effects of the two types of walks. Dogs had free catch urine samples taken multiple times per day for urinary cortisol analysis, were video recorded during walks, and were outfitted with activity monitoring collars. We found significant variation in cortisol levels in accordance with time of day, but no difference was found between the neighborhood and nature walks. Several stress, movement, and exploratory/foraging behaviors were found to differ between conditions. While we found that the experimental conditions did influence some of the dogs’ behavior in this study, they did not impact cortisol levels.
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    Dogs and wolves differ in their response allocation to their owner/caregiver or food in a concurrent choice procedure
    Isernia, Lindsay; Wynne, Clive D. L.; House, Leanna; Feuerbacher, Erica N. (PeerJ, 2022-02-15)
    Dogs and wolves both show attachment-like behaviors to their owners/caregivers, including exploring more in the presence of the owner/caregiver, and greeting the owner/caregiver more effusively after an absence. Concurrent choice studies can elucidate dogs' and wolves' relationship to their owners/caregivers by assessing their preference for the owner/caregiver compared to other stimuli. While previous research has used concurrent choice paradigms to evaluate dogs' and wolves' preference between humans giving social interaction or humans giving food, no research has explored their preferences for an owner/caregiver compared to food when the food is not delivered by a human. In the current study, we investigated whether dogs and hand-reared wolves preferred their owner/caregiver or food, unassociated with a human, when they had been equally deprived of each stimulus (at least 4 hours). Each canid experienced four trials; we measured first choice and time spent with each alternative. Dogs overall did not show a preference for the owner or food. Wolves, on the other hand, tended to show a preference for food in both measures. We observed a range of individual variation in both measures, although dogs showed more individual variation. The differences we observed between dogs and wolves align with prior research comparing wolf and dog behavior directed towards humans; however, the reasons for this differential responding could be due to a variety of factors beyond phylogeny.
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    Effects of Diet Change on Shelter Dog Kennel Behaviors and Cortisol Levels
    Boris, Samantha; Oberding, Natalie; Mercadante, Paula; Kerr, Anna; Andrukonis, Allison; Feuerbacher, Erica N. (Virginia Tech, 2024)
    Ketogenic diets have shown beneficial behavior effects as well as decreases in stress in mice and humans; however, this has not been empirically evaluated in dogs. The purpose of this study was to investigate if a ketogenic diet would be effective in reducing kennel reactivity, with a focus on barking, and cortisol levels in shelter dogs that currently display kennel reactivity. We placed 33 shelter dogs on a ketogenic diet over our nine-week study period (two weeks of a gradual diet change and four weeks on a full ketogenic diet). We collected one minute video recordings for the dogs twice every collection day for a total of eight videos over our four collection periods during Weeks 0, 3, 6, and 9. Of the 18 behaviors we analyzed, six significantly differed between the baseline and treatment phases. The positive behavior change we saw was a decrease in barking during treatment compared to baseline. Despite seeing a positive effect on barking, several affiliative behaviors observed in our study showed a significant decrease from baseline to treatment (facing forward, front of kennel, gazing, and wagging tail) while facing away increased during treatment. To assess the effect of diet on cortisol levels, we collected four fecal samples starting with Week 0 and then one every three weeks after during the nine-week study period. We found a significant decrease in fecal cortisol levels between baseline and treatment phases. In summary, we saw changes in cortisol levels to indicate decreases of stress when on a ketogenic diet, as well as a decrease in our main target behavior, barking. The results indicate something as simple as a change in diet is a feasible intervention for shelters to implement in real time to address in-kennel behavior issues, such as barking.
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    Effects of load type (pollen or nectar) and load mass on hovering metabolic rate and mechanical power output in the honey bee Apis mellifera
    Feuerbacher, Erica N.; Fewell, Jennifer H.; Roberts, Stephen P.; Smith, Elizabeth F.; Harrison, Jon F. (2003)
    In this study we tested the effect of pollen and nectar loading on metabolic rate (in mW) and wingbeat frequency during hovering, and also examined the effect of pollen loading on wing kinematics and mechanical power output. Pollen foragers had hovering metabolic rates approximately 10% higher than nectar foragers, regardless of the amount of load carried. Pollen foragers also had a more horizontal body position and higher inclination of stroke plane than measured previously for honey bees (probably nectar foragers). Thorax temperatures ranked pollen > nectar > water foragers, and higher flight metabolic rate could explain the higher thorax temperature of pollen foragers. Load mass did not affect hovering metabolic rate or wingbeat frequency in a regression-model experiment. However, using an analysis of variance (ANOVA) design, loaded pollen and nectar foragers (mean loads 27% and 40% of body mass, respectively) significantly increased metabolic rate by 6%. Mean pollen loads of 18% of body mass had no effect on wingbeat frequency, stroke amplitude, body angle or inclination of stroke plane, but increased the calculated mechanical power output by 16–18% (depending on the method of estimating drag). A rise in lift coefficient as bees carry loads without increasing wingbeat frequency or stroke amplitude (and only minimal increases in metabolic rate) suggests an increased use of unsteady power-generating mechanisms.
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    Effects of single- or pair-housing on the welfare of shelter dogs: Behavioral and physiological indicators
    Hecker, Grace; Martineau, Katherine; Scheskie, Mariah; Hammerslough, Rhonda; Feuerbacher, Erica N. (PLOS, 2024-06-12)
    Dogs are often housed alone in shelter settings to reduce injury and disease spread. However, social isolation can be a stressor for dogs. Prior studies have suggested that cohousing can produce behavioral and physiological benefits. These studies have typically focused on laboratory dogs or shelter dogs that have been kenneled for several months. Thus, those results might not necessarily generalize to shelter dogs, many of which have shorter lengths of stay than those dogs studied to date, and might be cohoused soon after intake. In fact, being pair-housed could, in the short term, be more stressful as dogs have to navigate novel social situations in small spaces. We investigated the behavioral and physiological effects of single- or pair-housing shelter dogs, most of which had recently entered the shelter. We collected behavioral data on 61 dogs (30 single-housed; 31 pair-housed) daily across seven days; we also collected urine for cortisol:creatinine analysis on a subset (22 single-housed; 18 pair-housed) for eight days (each day of the seven-day study plus a baseline sample on Day 0, prior to dogs’ enrollment). We found pair-housed dogs engaged in three stressrelated behaviors (lip licking, whining, and ears back) significantly less frequently than single- housed dogs. When we analyzed the change in urinary cortisol:creatinine (Days 1–7 values minus Day 0 value), we found that pair-housed dogs generally showed a greater decrease in cortisol:creatinine levels than single-housed dogs. Pair-housed dogs also had significantly shorter lengths of stay, but we did not detect any effect on dog-dog skills. Overall, we found well-matched pair-housing can have both proximate and ultimate welfare benefits for shelter dogs.
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    Efficacy of Wearable Therapies on the Ability to Improve Performance and Physical Health in Sport Horses
    Schmidt, Therese Elizabeth (Virginia Tech, 2023-04-25)
    Equines have been used for utilized for manual labor, recreation, and companionship amongst many other valuable conveniences since their domestication. As the modern horse progressed from livestock to athlete, attention was paid to the body conformation to be used as an indicator of biomechanics and can dictate equine performance. Poor conformation can put physical limitations on the body and predispose the horse to injury and chronic disease. When not managed properly, these flaws can lead to injury, lameness, and premature retirement in sport horses. The distal limb is composed of tendons and ligaments that are all susceptible to tear or rupture. Protective wraps or boots are typically applied to the distal limb prior to exercise to prevent superficial injury from the environment or interference. However, these preventatives can trap heat against the skin which can have detrimental effects on the fibroblasts which can lead to failure. It was not until the early twentieth century that the idea of equine physiotherapy was adopted, and practices changed to meet remedial needs and create a sustainable, healthy equine athlete. Equine physiotherapy is a broad-spectrum term used to describe the therapeutic efforts made to keep the body in good health by means of prevention of injury to improve or maintain performance. Traditionally, therapeutics are administered by a veterinarian or trained professional in the event of an existing injury. In recent years therapeutics have been commercialized and are readily available for everyday preventative use. The most common readily available treatments being variations of pulsating electromagnetic fields (PEMF), vibration therapy, cryotherapy, and thermotherapy. When used prior to or after exercise, the therapeutics are designed to prepare the body for exercise and improve recovery by increasing circulation and down regulating the inflammatory response. The studies performed evaluate the efficacy of Rambo®Ionic (Horseware, Dundalk,Ireland), Lux Ceramic Therapy® (Schneider Saddlery Co., Inc., Ohio, USA), and Ice-Vibe® (Horseware, Dundalk,Ireland) therapeutic boots when applied to the distal limb as per manufacturer recommendation. The first study evaluated the therapeutic boots ability to alter performance performing gait analysis using the ALOGO™ MovePro (Alogo Technologies, Switzerland) stride sensor, blood analysis measuring serum concentrations of C reactive protein (CRP), basic fibroblast growth factor (bFGF) and tenascin-C (TN-C), and capturing thermal images of the distal limb using an HT-19 thermal imaging camera (HTI, La Vergne, TN). In this study, eight healthy horses were exercised for approximately ten minutes per day for five consecutive days. There was a ten-day washout period where the horse received no treatment between each period; there was a total of four periods. The second study only evaluated Rambo®Ionic (Horseware, Dundalk,Ireland) and Ice-Vibe® (Horseware, Dundalk,Ireland) therapeutic boots on seventeen healthy horses in the Virginia Tech equitation lesson program. There were three periods with five days of consecutive data collection and a ten-day washout period in between where the horses received no treatment. Gait analysis was measured using the ALOGO™ MovePro (Alogo Technologies, Switzerland) stride sensor and a blind behavioral analysis was performed to analyze behavioral changes under saddle in response to a rider.
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    Emergency Fostering of Dogs From Animal Shelters During the COVID-19 Pandemic: Shelter Practices, Foster Caregiver Engagement, and Dog Outcomes
    Gunter, Lisa M.; Gilchrist, Rachel J.; Blade, Emily M.; Reed, Jenifer L.; Isernia, Lindsay T.; Barber, Rebecca T.; Foster, Amanda M.; Feuerbacher, Erica N.; Wynne, Clive D. L. (Frontiers, 2022-04-27)
    Each year, millions of dogs enter thousands of animal shelters across the United States. Life in the shelter can be stressful, and one type of intervention that improves dogs' experience is human interaction, particularly stays in foster homes. Prior research has demonstrated that fostering can reduce dogs' cortisol and increase their resting activity. Despite these benefits, little is understood about the utilization of foster caregiving in animal shelters, and even less so during a crisis. On March 11, 2020, the World Health Organization deemed the coronavirus outbreak a worldwide pandemic, and subsequently a nationwide emergency was declared in the United States. Nearly all states issued stay-at-home orders to curb the spread of the virus. During this time, media outlets reported increased interest in the adoption and fostering of shelter pets. This study explores canine foster caregiving at 19 US animal shelters during the first 4 months of the COVID-19 pandemic. In our investigation, we found that shelters' utilization of foster caregiving increased from March to April 2020 but returned to initial pandemic levels by June 2020. Slightly less than two-fifths of foster caregivers were community members with no prior relationship with the shelter, and these caregivers were over four times more likely to adopt their fostered dogs than those with a pre-existing relationship to the shelter. Individuals fostering with the intention to adopt, in fact, adopted their dogs in nearly three-quarters of those instances. With regards to shelters' available resources, we found that very low-resource shelters relied more heavily on individuals with prior relationships to provide foster caregiving while very high-resource shelters more often recruited new community members. We also found that our lowest resourced shelters transferred more dogs out of their facilities while more resourced shelters rehomed dogs directly to adopters. To our knowledge, these findings represent the first in-depth reporting about dog fostering in US animal shelters and, more specifically, foster caregiving during the COVID-19 pandemic. In total, they provide greater understanding of how monetary and human resources were utilized to affect the care and ultimately, the outcomes of shelter dogs during this time.
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    Epigenetic and Ubiquitin-Proteasome Mechanisms of Obesity Development
    McFadden, Taylor Marie (Virginia Tech, 2023-04-14)
    Obesity is a major health condition in which little is known about the molecular mechanisms that drive it. The hypothalamus is the primary control center for controlling both food intake and energy expenditure in order to maintain the body's energy balance and dysregulation of molecular processes in this region have been implicated in the development and progression of obesity. Recently, several studies have shown altered DNA methylation of critical appetite genes, including the satiety gene Pomc, in the hypothalamus of rodents fed a high fat obesogenic diet. However, it has not previously been studied whether diet-induced changes in DNA methylation of critical appetite genes in the hypothalamus contributes to the development and persistence of the obesity phenotype. Further, DNA 5-hydroxymethylation (5-hmC) is one type of DNA methylation that is 10 times more abundant in the brain than peripheral tissues. However, to date, no study has been conducted examining whether DNA 5-hmC becomes altered in the brain following weight gain and/or contributes to the obesity phenotype. Additionally, there is also evidence to support that exposure to a high fat diet dysregulates the activity of the ubiquitin-proteasome system, the master regulator of protein degradation in cells, in the hypothalamus of male rodents. Despite this, whether this can occur in both sexes and directly contributes to abnormal weight gain has not been investigated. Here, we used a rodent diet-induced obesity model in combination with quantitative molecular assays and CRISPR-dCas9 manipulations to test the role of hypothalamic 1) DNA 5-hmC levels, 2) Pomc methylation, and 3) dysregulated ubiquitin-proteasome signaling in abnormal weight gain following exposure to obesogenic diets. We found that males, but not females, have decreased levels of DNA 5-hmC in the hypothalamus following exposure to a high fat diet, which tracked body weight. Short-term exposure to a high fat diet, which does not result in significant weight gain, resulted in decreased hypothalamic DNA 5-hmC levels, suggesting these changes occur prior to obesity development. Moreover, decreases in DNA 5-hmC persist even after the high fat diet is removed. Importantly, CRISPR-dCas9 mediated upregulation of DNA 5-hmC enzymes in the male, but not female, hypothalamus significantly reduced the percentage of weight gained on the high fat diet relative to controls. Next, we used the CRISPR-dCas9-TET1 and dCas9-DNMT3a systems to test the role of Pomc DNA methylation in the hypothalamus in abnormal weight gain following acute exposure to a high fat diet in male rats. We found that exposure to a high fat diet increases Pomc DNA methylation and reduces gene expression in the hypothalamus. Despite this, we found that CRISPR-dCas9-TET1-mediated demethylation of Pomc was not sufficient to prevent abnormal weight gain following exposure to a high fat diet. Moreover, CRISPR-dCas9-DNMT3a-mediated methylation of Pomc did not alter weight gain following exposure to standard or high fat diets. Finally, we found that both males and females showed dynamic downregulation of proteasome activity, decreases in proteasome subunit expression and an accumulation of degradation-specific K48 polyubiquitinated proteins in the hypothalamus. However, while the CRISPR-dCas9 system was able to selectively increase some forms of proteasome activity, it was unable to prevent diet-induced proteasome downregulation or abnormal weight gain. Collectively, this data reveals novel, sex-specific differences in the engagement of the ubiquitin proteasome system and role of DNA 5-hydroxymethylation in the hypothalamus during the development of the obesity phenotype.
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    Evaluating the effects of a temporary fostering program on shelter dog welfare
    Gunter, Lisa M.; Feuerbacher, Erica N.; Gilchrist, Rachel J.; Wynne, Clive D. L. (PeerJ, 2019-03-27)
    One of the greatest stressors for dogs living in animal shelters is social isolation. Many studies have demonstrated that human interaction reduces cortisol in shelter dogs, with the possibility that longer periods of interaction may yield greater effects. These types of interventions are contingent upon removing the dog from the kennel and any such reductions in cortisol are often lost when the dog returns to the kennel. More recently, animal shelters are utilizing short-term fostering programs to provide relief from the perceived stresses of kennel life; however the effects of these programs are not well understood. This study assessed the impacts of one-and two-night fostering programs on the urinary cortisol levels, resting pulse rates, longest bout of uninterrupted rest, and proportion of time spent resting of dogs awaiting adoption. Five animal shelters, open and limited-admission facilities, from across the United States participated in the study. During the study, dogs' urine was collected in the morning before, during, and after fostering stays for cortisol: creatinine analysis. Non-invasive health monitors were worn by the dogs, which collected heart rates and activity levels, in the shelter and in foster homes. In total, 207 dogs participated in the study, and 1,076 cortisol values were used in our analysis. Across all shelters, we found that dogs' cortisol: creatinine ratios dropped significantly during their fostering stay, but returned to baseline levels after return to the shelter. However, the observed reduction in cortisol varied in magnitude across shelters. We found that dogs of greater weight, age, and average resting pulse rate had higher cortisol levels; and dogs with longer bouts of uninterrupted rest had lower cortisol levels. Dogs had their longest bouts of rest during sleepovers, followed by in the shelter after their sleepovers. Lastly, significant differences were found when comparing in-shelter cortisol values at our five shelters, differences that were in some cases greater than the impact of the fostering intervention itself. Considering the diversity of facilities that participated in this study, it is possible that as yet unstudied, shelter-specific, environmental factors could be contributing to the overall welfare of shelter dogs. Thus while a reprieve from the shelter is impactful for dogs awaiting adoption, mitigating the stressors present in kenneling conditions should also be addressed to improve the lives of shelter dogs.
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    Exploring the Domestication Syndrome Hypothesis in Dogs: Pigmentation Does Not Predict Cortisol Levels
    Platzer, JoAnna M.; Gunter, Lisa M.; Feuerbacher, Erica N. (MDPI, 2023-10-04)
    Previous research has found connections between pigmentation, behavior, and the physiological stress response in both wild and domestic animals; however, to date, no extensive research has been devoted to answering these questions in domestic dogs. Modern dogs are exposed to a variety of stressors; one well-studied stressor is residing in an animal shelter. To explore the possible relationships between dogs’ responses to stress and their pigmentation, we conducted statistical analyses of the cortisol:creatinine ratios of 208 American shelter dogs as a function of their coat color/pattern, eumelanin pigmentation, or white spotting. These dogs had been enrolled in previous welfare studies investigating the effect of interventions during which they left the animal shelter and spent time with humans. In the current investigation, we visually phenotype dogs based on photographs in order to classify their pigmentation and then conduct post hoc analyses to examine whether they differentially experience stress as a function of pigmentation. We found that the dogs did not differ significantly in their urinary cortisol:creatinine ratios based on coat color/pattern, eumelanin pigmentation, or white spotting, either while they were residing in the animal shelter or during the human interaction intervention. These preliminary data suggest that pigmentation alone does not predict the stress responses of shelter dogs; however, due to the small sample size and retrospective nature of the study, more research is needed.
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    Factors Associated with Behavioral Euthanasia in Pet Dogs
    Hitchcock, Miranda (Virginia Tech, 2023-02-10)
    When pet dogs demonstrate unwanted or problematic behaviors, particularly severe behaviors, owners may choose to euthanize. While the topic of behavioral euthanasia is becoming more common among dog behavior experts, shelters, and rescues, there has been little research in this area. The Behavioral Euthanasia in Pet Dogs Questionnaire was developed to gather detailed information about dogs that were euthanized for behavior reasons. This exploratory study found that aggression, particularly aggression toward people, was the most common behavior that led to euthanasia. Results indicated that behavioral euthanasia occurs across a wide age range of dogs, and that most of these dogs had been living in their home and exhibiting the problem behavior for a long time prior to euthanasia. Additionally, reports of bites and self-harm indicate the severity of the problematic behaviors that led to euthanasia. This research provides a foundation for future study and provides an overview of the behavioral factors associated with behavioral euthanasia.
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    Going to the Vet? Don’t Fret: Using Treats and Scale Mats to Promote a “Stress-Free” Veterinary Experience for Dogs
    Score, Tiffany (Virginia Tech, 2024-05-08)
    Over 76.8 million pet dogs live in the United States and visit a veterinary clinic 2.4 times yearly, on average. For most dogs, these vet visits evoke stress, adding complications and complexity to executing necessary and routine care procedures. In a two-by-two mixed factorial study, we explored the potential effects and interactions relating to how a dog gets onto a veterinary scale, using a food lure or physical prompt, and whether a scale mat impacts procedural efficacy or efficiency. To analyze behavioral stress indicators within a veterinary context, we video-recorded a 3-min pre-experimental waiting period for each participant under both conditions. Dogs mounted the scale significantly faster if the scale was covered in a non-slip mat and a food lure was used. The handler effect was also significant, revealing that scale mounting procedural efficiency was impacted by whom the dog was handled. Lastly, behavioral analyses highlighted some key, easy-to-identify stress-related behavioral indicators that could serve as early warning signs a dog may struggle to complete necessary routine veterinary care procedures. These findings suggest simple, cost-effective strategies to reduce a dog’s stress when visiting the veterinary clinic.
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    The Great Outdoors: Fenced Yards and Their Impact on Companion Dog Activity and Adoption
    Tsao, Lauren E.; Feuerbacher, Erica N.; Hoffman, Christy L.; Andrukonis, Allison (Virginia Tech, 2022-06-30)
    Despite the prevalence of requiring a fenced yard to adopt a dog in the United States, there is little research investigating this adoption policy or its impact on companion dog welfare and behavior. Using observational methods, previous studies have shown dogs engage in a variety of behaviors while in their yards. However, differences in their activity levels when they are in the yard versus when they are not have not been measured through more objective means. In this study, I first conducted an analysis of publicly available data from the pet adoption website, PetFinder.com to show how many United States rescues/shelters are requiring fenced yards to adopt a dog. In the second part of the study, I fitted AX3 accelerometers to 12 companion dogs with regular fenced yard access to investigate the activity levels of dogs when in a fenced yard and when not in a fenced yard. The results indicate that shelters and rescues required fenced yards for dog adoption in over 20% of the United States adoption profiles analyzed and that fenced yard access increased dog activity level in some dog participants.
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    Habituation Protocols Improve Behavioral and Physiological Responses of Beef Cattle Exposed to Students in an Animal Handling Class
    Ujita, Aska; Seekford, Zachary; Kott, Michelle L.; Goncherenko, Guillermo; Dias, Nicholas W.; Feuerbacher, Erica N.; Bergamasco, Luciana; Jacobs, Leonie; Eversole, Dan Edward; Negrão, João A.; Mercadante, Vitor R. G. (MDPI, 2021-07-21)
    Our objective was to determine the impact of different habituation protocols on beef cattle behavior, physiology, and temperament in response to human handling. Beef heifers were exposed to three habituation strategies: (1) tactile stimulation (brushing) in the working chute for seven consecutive days (STI; n = 18); (2) passage through the working chute for seven consecutive days (CHU; n = 19) and; (3) no habituation (CON; n = 19). Individual heifer respiratory rate (RR; n/min), internal vaginal temperature (VAGT; °C), and blood cortisol were measured. Further, behavior parameters were observed to generate a behavior score, and heifer interaction with students and their behavioral responses were recorded. Habituation with STI and CHU resulted in improved numerical behavioral scores compared to CON, and greater (p ≤ 0.05) handling latencies. Vaginal temperature was decreased in STI compared to CHU and CONT (p ≤ 0.05). Cortisol concentration did not differ among treatments, but decreased (p ≤ 0.05) from the start of the experiment to 14 days after treatment initiation. Both habituation protocols showed benefits, but heifers that received the positive tactile stimulation in the chute had the greatest behavior improvements. Furthermore, these heifers responded more calmly during student-animal interactions in class, which is beneficial for the students’ and animals’ safety.
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    A History of Dogs as Subjects in North American Experimental Psychological Research
    Feuerbacher, Erica N.; Wynne, Clive D. L. (2011)
    The modern resurgence in psychological experiments involving dogs follows a long and rich tradition of using dogs as experimental subjects in psychology. Except for a few exceptions (e.g., Pavlov, and Scott and Fuller), much of this research is often overlooked. We trace the history of dogs as experimental psychological subjects: The work of Darwin and Pavlov sets the stage for our focus on research emanating from North American laboratories. We end our review with the advent of the modern renaissance of dog research. This account tracks the history of psychology as a science, providing insight into psychological processes and theoretical corollaries of these processes generally, and shedding light on the behavior of dogs specifically. A rediscovery of this literature can only aid research being conducted today, including rejuvenating old ques¬tions, suggesting new ones, and highlighting useful methods for current issues. We hope through this endeavor that those working with dogs will see themselves as part of this rich tradition and that a historical perspective will help integrate dog research into a field greater than the sum of its parts.