Browsing by Author "Gopalakrishnan, Mahima"
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- Effects of receptor clustering on ligand dissociation kinetics: Theory and simulationsGopalakrishnan, Mahima; Forsten-Williams, Kimberly; Nugent, M. A.; Täuber, Uwe C. (Cell Press, 2005-12-01)
- Effects of receptor clustering on ligand dissociation kinetics: Theory and simulationsGopalakrishnan, Mahima; Forsten-Williams, Kimberly; Nugent, Matthew A.; Täuber, Uwe C. (Cell Press, 2005-12-01)Receptor-ligand binding is a critical first step in signal transduction and the duration of the interaction can impact signal generation. In mammalian cells, clustering of receptors may be facilitated by heterogeneous zones of lipids, known as lipid rafts. In vitro experiments show that disruption of rafts significantly alters the dissociation of fibrbroblast growth factor-2 (FGF2) from heparan sulfate proteoglycans (HSPGs), co-receptors for FGF-2. In this article, we develop a continuum stochastic formalism to address how receptor clustering might influence ligand rebinding. We find that clusters reduce the effective dissociation rate dramatically when the clusters are dense and the overall surface density of receptors is low. The effect is much less pronounced in the case of high receptor density and shows nonmonotonic behavior with time. These predictions are verified via lattice Monte Carlo simulations. Comparison with FGF-2-HSPG experimental results is made and suggests that the theory could be used to analyze similar biological systems. We further present an analysis of an additional cooperative internal-diffusion model that might be used by other systems to increase ligand retention when simple rebinding is insufficient.
- File Formats, Transformation, and MigrationLeidig, Jonathan; Alon, A. J.; Chigani, Amine; Gopalakrishnan, Mahima; Park, Sung Hee (2009-10-09)This module covers the principles and applications of the transformation and migration processes for the preservation of digital content, as well as key issues surrounding digital preservation strategies.
- Ligand rebinding: self-consistent mean-field theory and numerical simulations applied to surface plasmon resonance studiesGopalakrishnan, Mahima; Forsten-Williams, Kimberly; Cassino, T. R.; Padro, L.; Ryan, T. E.; Täuber, Uwe C. (Springer, 2005-10-01)
- Ligand-induced coupling versus receptor pre-association: cellular automaton simulations of FGF-2 bindingGopalakrishnan, Mahima; Forsten-Williams, Kimberly; Täuber, Uwe C. (Academic Press – Elsevier, 2004-03-21)