Browsing by Author "Haikal, Amr M."

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    Diversity in blueberry genotypes and developmental stages enables discrepancy in the bioactive compounds, metabolites, and cytotoxicity
    Das, Protiva Rani; Darwish, Ahmed G.; Ismail, Ahmed; Haikal, Amr M.; Gajjar, Pranavkumar; Balasubramani, Subramani Paranthaman; Sheikh, Mehboob B.; Tsolova, Violeta; Soliman, Karam F. A.; Sherif, Sherif M.; El-Sharkawy, Islam (Elsevier, 2022-04-16)
    Eight blueberry cultivars at three developmental stages were investigated for metabolite profiling, antioxidant, and anticancer activities. Cultivars- and developmental stages-variations were determined in total phenolic, flavonoid, DPPH, and FRAP antioxidant assays. The anticancer capacity was equal against A549, HepG2, and Caco-2 cancer cells, whereas the inhibition rate was dose-, incubation period-, cultivar-, and developmental stages-dependent. The untargeted metabolite profiling by UPLC-TOF-MS analysis of two contrast cultivars, 'Vernon' and 'Star', throughout the developmental stages revealed 328 metabolites; the majority of them were amino acids, organic acids, and flavonoids. The multivariate statistical analysis identified five metabolites, including quinic acid, methyl succinic acid, chlorogenic acid, oxoadipic acid, and malic acid, with positively higher correlations with all anticancer activities. This comprehensive database of blueberry metabolites along with anticancer activities could be targeted as natural anticancer potentials. This study would be of great value for food, nutraceutical, and pharmaceutical industries as well as plant biotechnologists.
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    Transcriptome Profiling of a Salt Excluder Hybrid Grapevine Rootstock ‘Ruggeri’ throughout Salinity
    Gajjar, Pranavkumar; Ismail, Ahmed; Islam, Tabibul; Moniruzzaman, Md; Darwish, Ahmed G.; Dawood, Ahmed S.; Mohamed, Ahmed G.; Haikal, Amr M.; El-Saady, Abdelkareem M.; El-Kereamy, Ashraf; Sherif, Sherif M.; Abazinge, Michael D.; Kambiranda, Devaiah; El-Sharkawy, Islam (MDPI, 2024-03-14)
    Salinity is one of the substantial threats to plant productivity and could be escorted by other stresses such as heat and drought. It impairs critical biological processes, such as photosynthesis, energy, and water/nutrient acquisition, ultimately leading to cell death when stress intensity becomes uncured. Therefore, plants deploy several proper processes to overcome such hostile circumstances. Grapevine is one of the most important crops worldwide that is relatively salt-tolerant and preferentially cultivated in hot and semi-arid areas. One of the most applicable strategies for sustainable viticulture is using salt-tolerant rootstock such as Ruggeri (RUG). The rootstock showed efficient capacity of photosynthesis, ROS detoxification, and carbohydrate accumulation under salinity. The current study utilized the transcriptome profiling approach to identify the molecular events of RUG throughout a regime of salt stress followed by a recovery procedure. The data showed progressive changes in the transcriptome profiling throughout salinity, underpinning the involvement of a large number of genes in transcriptional reprogramming during stress. Our results established a considerable enrichment of the biological process GO-terms related to salinity adaptation, such as signaling, hormones, photosynthesis, carbohydrates, and ROS homeostasis. Among the battery of molecular/cellular responses launched upon salinity, ROS homeostasis plays the central role of salt adaptation.