Browsing by Author "Hrubec, T. C."

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    Hematology and Blood Chemistry Reference Intervals for Yellow Perch (Perca flavescens) Raised in Recirculation Systems
    Hrubec, T. C.; Smith, Stephen A. (Commercial Fish and Shellfish Technologies Program, Virginia Tech, 2004-06-01)
    Determination of hematology and blood plasma biochemistry values is routinely used to assess the health of wild and domestic animals. Yellow perch (Perca flavescens) culture is a growing segment of the U.S. aquaculture industry and tools are needed to monitor the health status of these fish. This paper reports reference values for complete hematological and biochemistry profiles of normal, healthy yellow perch raised in recirculation culture conditions. The following hematologic values were determined: packed cell volume, plasma protein, erythrocyte, leukocyte, lymphocyte, neutrophil, monocyte, and thrombocyte numbers. A description of leukocyte morphology is presented. Additionally, the following plasma biochemical values were determined: total protein, albumin, globulin, creatinine, total bilirubin, alkaline phosphatase, aspartate aminotransferase, sodium, potassium, chloride, calcium, phosphorus, magnesium, glucose, and cholesterol. Reference values for a specific population of fish need to be determined prior to utilizing diagnostic blood samples from individuals. Developing diagnostic hematology for fishes can enhance yellow perch culture by providing a means for the early detection and identification of infectious disease and of sub-lethal conditions that may affect production performance.
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    Hematology and Plasma Chemistry Values for Production Tilapia (Oreochromis hybrid) Raised in a Recirculation System
    Hrubec, T. C.; Smith, Stephen A. (Commercial Fish and Shellfish Technologies Program, Virginia Tech, 2000-06-01)
    Tilapia are a frequently aquacultured fish, yet little is known about their normal physiology and response to disease. To assess specific diseases in mammals, blood constituents are routinely analyzed and compared to previously determined standardized values. Research to establish hematologic values for normal healthy fish is required before blood analysis can be used for diagnostic purposes in fishes. This study determined hematology and plasma chemistry values for production tilapia (Oreochromis hybrids) raised in a recirculation system. Using standard clinical techniques, the following hematologic parameters were determined: PCV (packed cell volume), plasma protein and MCV (mean cell volume) values; and erythrocyte, leukocyte, lymphocyte (small and large), neutrophil, monocyte, eosinophil, thrombocyte-like-cell, and thrombocyte numbers. Additionally, the following plasma chemistry values were determined: total protein, albumin, globulin, creatinine, total bilirubin, ALP (alkaline phosphatase), AST (aspartate aminotransferase), sodium, potassium, chloride, calcium, phosphorus, magnesium, glucose, cholesterol, ammonia and osmolality. Analysis of blood parameters can enhance production of hybrid tilapia by providing a means for the early detection of infectious diseases, and by assisting in the identification of sub-clinical conditions affecting production performance.
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    Hematology and Serum Chemistry Values for Winter Flounder (Pleuronectes americanus)
    Dye, V. A.; Hrubec, T. C.; Dunn, J. L.; Smith, Stephen A. (Commercial Fish and Shellfish Technologies Program, Virginia Tech, 2001-06-01)
    Clinical analysis of blood to determine hematology and plasma biochemistry values is routinely used to assess the health of wild and domestic animals. Flounder culture is a fast growing segment of the U.S. aquaculture industry and tools are needed to monitor the health of these fish. This paper reports a complete hematologic and blood biochemistry profile for normal healthy winter flounder, Pleuronectes americanus, maintained in recirculated artificial seawater. The following hematologic values were determined: Packed cell volume, plasma protein, erythrocyte number, hemoglobin, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, and leukocyte, lymphocyte, neutrophil, monocyte, and thrombocyte numbers. A description of leukocyte morphology is presented. Additionally, the following serum biochemical values were determined: Total protein, albumin, globulin, sodium, potassium, chloride, calcium, phosphorus, magnesium, glucose, blood urea nitrogen, creatinine, total bilirubin, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, cholesterol and triglycerides. Analysis of blood parameters can enhance flounder culture by providing a means for the early detection and identification of infectious disease and of sub-lethal conditions affecting production performance.