Browsing by Author "Hurt, Christopher"
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- Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mappingTabor, Kathryn M.; Marquart, Gregory D.; Hurt, Christopher; Smith, Trevor S.; Geoca, Alexandra K.; Bhandiwad, Ashwin A.; Subedi, Abhignya; Sinclair, Jennifer L.; Rose, Hannah M.; Polys, Nicholas F.; Burgess, Harold A. (2019-02-08)Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.