Browsing by Author "Jackson, Bryan Tyler"

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    La Crosse Virus in Southwestern Virginia: Role of Exotic Mosquito Species and Effect of Virus Infection on Feeding
    Jackson, Bryan Tyler (Virginia Tech, 2009-05-29)
    The family Bunyaviridae is the largest of vertebrate diseases and includes the mosquito-borne disease La Crosse (LAC) virus. Vectors include the major vector Aedes triseriatus and two accessory vectors Aedes albopictus and Aedes japonicus. In the past several decades there has been an increase in the number of LAC cases, implication of new vectors, and the appearance of new foci of disease in the southeastern U.S. To better understand the vectors and the relationship between vectors and the virus, laboratory and field studies were conducted to determine oviposition preferences, effect of virus infection on blood-feeding behavior, and compare the efficacy of various methods to determine minimum infection rates of vectors. In laboratory studies of oviposition preference, only Ae. japonicus demonstrated a preference when presented with preexisting eggs. They deposited more eggs in cups containing either conspecifics or Ae. albopictus. The presence of 1st instar larvae Ae. albopictus larvae deterred oviposition by Ae. triseriatus and Ae. japonicus. Ae. japonicus and Ae. triseriatus preferred cups containing larval rearing water (LRW) of conspecifics and Ae. albopictus. Aedes albopictus preferred LRW regardless of species compared to control cups. Field experiments with fresh egg papers and preexisting eggs did not show significant differences, although the unequal population densities of species in the study area confounded the analysis. More work is needed to elucidate the interaction among these species and its effect on oviposition in the field. Blood-feeding experiments showed that LAC virus-infected Ae. triseriatus and Ae. albopictus imbibed significantly less blood compared to uninfected mosquitoes. Because blood meal size affects the subsequent inhibition of host seeking, experiments were done to ascertain the effect of virus infection on refeeding. Significantly more infected Ae. triseriatus mosquitoes refed but there was no effect on the refeeding rate of Ae. albopictus. Thus, the detrimental effect of virus infection, i.e., reduction in blood meal size, may lead to increased host exposure by Ae. triseriatus, enhancing horizontal transmission. Collecting adult mosquitoes was more efficient to detect virus in field populations than the collection of eggs. Maximum likelihood estimation-infection rates (MLE-IR) were calculated using bias-corrected maximum likelihood estimation. Adult collections yielded significantly more positive pools compared with egg collections. Virus was isolated from pools from Ae. canadensis, Ae. triseriatus, and Ae. albopictus. These results are comparable to other studies.
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    Oviposition Preferences for Infusion-Baited Traps and Seasonal Abundance of Culex Mosquitoes in Southwestern Virginia
    Jackson, Bryan Tyler (Virginia Tech, 2004-07-19)
    Field studies were conducted in southwestern Virginia to determine the bionomics and ovipositional preferences of Culex restuans Theobald and Culex pipiens Linnaeus using ovitraps and gravid traps. Both species have been implicated as enzootic and epizootic vectors of West Nile virus (WNV) and these studies provide information on the relative abundance of gravid mosquitoes. Ovitraps were used in the summers of 2002 and 2003 to measure the oviposition activity of Culex mosquitoes, mainly Cx. restuans and Cx. pipiens. In 2002, 1,345 egg rafts were collected from 5 traps set at different locations in the New River Valley (NRV). Cx. restuans constituted 93.2% of the catch; the remainder was Cx. pipiens (6.7%) and Cx. salinarius (<1%). In 2003, 4 ovitraps were placed at each of 6 locations in the NRV. Of 9,794 egg rafts collected, Cx. restuans comprised 92.8%, Cx. pipiens 6.5%, and Cx. salinarius <1%. Oviposition patterns were similar in both years. Cx. restuans oviposition was detected about mid-May, and raft numbers were highest in late June and the middle of July and then showed a steady decline throughout the remainder of each season. Cx. pipiens oviposition activity began later in the season and gradually increased, reaching its peak in August. Although the number of egg rafts of Cx. restuans decreased in August and September while the number of Cx. pipiens egg rafts increased, a crossover in the relative abundance of the two species never occurred. In 2003, the attractiveness of four infusions (cow manure, straw, grass, and rabbit chow) were compared in oviposition traps. For Cx. restuans, the manure infusions collected more egg rafts than the other three infusions for the first four weeks, with two of the weeks showing significance. During week 1, the manure infusions collected significantly more egg rafts than straw (P<0.01), grass (P<0.01), and rabbit (P<0.001). During week 2, manure collected more than grass (P<0.05) and rabbit (P<0.01). The straw and grass infusions yielded the most egg rafts after week 2, and only three weeks showed any significance. When traps began to collect Cx. pipiens, the majority were collected in the straw and grass infusions. During week 6, the hay infusions had significantly more egg rafts when compared to manure (P<0.001) and rabbit infusions (P<0.001) and the grass infusions had significantly more when compared to manure (P<0.05) and rabbit (P<0.01). Week 9 also showed significance when the hay infusions collected significantly more egg rafts than manure and rabbit (P<0.01 and P<0.01, respectively). The attractiveness of the cow manure and straw infusions were also compared in gravid traps. Because it is difficult to accurately discriminate between Cx. pipiens and Cx. restuans that have been collected as adults in gravid traps, these collections were combined into Culex. More Culex mosquitoes were collected in the manure infusions for the first two weeks (P<0.05). No significant differences were found between the numbers of mosquitoes captured in the traps baited with the different infusions after the second week. The shift observed in oviposition preference for both types of traps may have been due to cooler temperatures in the early part of the season. The straw infusions were aged outdoors for 3 days prior to use, and a sufficient incubation temperature to generate the bacteria producing the attractant chemicals may not have been attained. In addition, the manure lagoon had been drawn down and the consistency of the remaining manure became much drier by this time. No chemicals were ever added to the lagoon, but the draw down may have affected the attractiveness of the manure.