Browsing by Author "Klopf, Sara K."
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- Developing Field and Lab Predictors of Appalachian Surface Coal Mine Spoil Leachate GeochemistryJohnson, Daniel K.; Daniels, W. Lee; Orndorff, Zenah W.; Ross, L. Clay; Klopf, Sara K. (Virginia Tech. Powell River Project, 2015)
- Effects of Glyphosate Herbicide on Phytophthora cinnamomi and Mine Soil Microbial CommunitiesKlopf, Sara K.; Holliday, Jason A.; Badgley, Brian D. (Virginia Tech. Powell River Project, 2019)The American Chestnut (Castanea denata) was once the dominant hardwood species within the forests of the Appalachians and an important resource for people and wildlife. In the early 1900s, a fungal blight (Cryphonectria parasitica) was introduced from imported ‘Japanese Giant’ nursery trees that caused topkill of American chestnuts (Tallamy 2007). Trees infected with C. parasitica die back, then continually resprout from the roots. Since 1983, the American Chestnut Foundation has been conducting a backcross breeding program to produce hybrid trees resistant to the blight, and more recently, various research institutions have been working to develop transgenic varieties of American chestnut which express genes that may give the trees resistance to the blight. Additional pathogens, such as the fungus-like oomycete which causes root rot (Phytophthora cinnamomi), have furthered threatened C. dentata, and research of transgenic American chestnuts has included the identification of genes that may provide resistance to P. cinnamomi.
- Establishing Hardwood Forests on Appalachian Mine Sites Using the Forestry Reclamation ApproachZipper, Carl E.; Klopf, Sara K.; Krenz, Robert J.; Evans, Daniel M.; Burger, James A.; Fields-Johnson, Chris W. (Virginia Tech. Powell River Project, 2016)
- Long-Term Effects of Rock Type, Amendments and Weathering on Mine Soil Properties and TDS PotentialsDaniels, W. Lee; Johnson, Daniel K.; Haering, Kathryn; Klopf, Sara K.; Angel, Hannah; Burger, Julia C. (Virginia Tech. Powell River Project, 2018)
- Long-Term Effects of Rock Type, Amendments and Weathering on Mine Soil Properties and TDS PotentialsDaniels, W. Lee; Johnson, Daniel K.; Haering, Kathryn; Klopf, Sara K.; Burger, Julia C. (Virginia Tech. Powell River Project, 2017)
- Long-Term Effects of Rock Type, Amendments and Weathering on Mine Soil Properties and TDS PotentialsDaniels, W. Lee; Angel, Hannah; Stewart, Ryan D.; Klopf, Sara K.; Johnson, Daniel K.; Haering, Kathryn (Virginia Tech. Powell River Project, 2021-02-01)
- Survival, Growth, and Blight Incidence of Pure and Hybrid American Chestnuts After Nine Growing Seasons on a FRA-Reclaimed Coal MineKlopf, Sara K.; Holliday, Jason A. (Virginia Tech. Powell River Project, 2017)
- Toward Deregulation of Genetically Modified American Chestnut for Surface Mine Reclamation: Genomic Mechanisms of Transgene-Mediated Blight ResistanceHolliday, Jason A.; Klopf, Sara K. (Virginia Tech. Powell River Project, 2018)We have been testing numerous candidate genetically modified (GM) lines of American chestnut (Castanea dentata) at the Powell River Project since 2014, with several showing promising levels of resistance and growth. Former surface mines represent an obvious context in which to begin restoration efforts using these GM trees, but extensive data on the environmental and ecological impacts of the transgenes is required before the US Department of Agriculture will consider their deployment outside of our current heavily monitored field trials. One of the first such pieces of data will be to show at the cellular level how a given transgene leads to resistance. As genes and their protein products function in regulatory networks that may involve thousands of other genes/proteins, assessing the risk (or lack thereof) when a transgene escapes requires an understanding of how that transgene functions in the tree, and whether it has undesirable off-target effects. One way to address this question is to leverage next generation sequencing to assess how gene regulatory networks are perturbed in the GM vs. wild-type lines. In this study, we have inoculated a number of trees in our GM field trial with Cryphonectria parasitica (chestnut blight) and sampled the resulting cankers for extraction of RNA. RNA-sequencing work is on-going, but we will achieve a detailed view of how the transgenes affect the rest of the gene regulatory network in these trees (that is, which genes are turned on/off and to what degree). The data arising from this project will supplement ongoing studies by collaborators toward the ultimate goal of de-regulation for one or more of these GM lines, and more extensive deployment of the trees for surface mine reclamation. Here we present some preliminary results from this study.