Browsing by Author "Liu, Ning"
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- Control of mitochondrial metabolism and systemic energy homeostasis by microRNAs 378 and 378*Carrer, Michele; Liu, Ning; Grueter, Chad E.; Williams, Andrew H.; Frisard, Madlyn I.; Hulver, Matthew W.; Bassel-Duby, Rhonda; Olson, Eric N. (NAS, 2012-09-18)Obesity and metabolic syndrome are associated with mitochondrial dysfunction and deranged regulation of metabolic genes. Peroxisome proliferator-activated receptor γ coactivator 1β (PGC-1β) is a transcriptional coactivator that regulates metabolism and mitochondrial biogenesis through stimulation of nuclear hormone receptors and other transcription factors. We report that the PGC-1β gene encodes two microRNAs (miRNAs), miR-378 and miR-378*, which counterbalance the metabolic actions of PGC-1β. Mice genetically lacking miR-378 and miR-378* are resistant to high-fat diet-induced obesity and exhibit enhanced mitochondrial fatty acid metabolism and elevated oxidative capacity of insulin-target tissues. Among the many targets of these miRNAs, carnitineO-acetyltransferase, a mitochondrial enzyme involved in fatty acid metabolism, and MED13, a component of the Mediator complex that controls nuclear hormone receptor activity, are repressed by miR-378 and miR-378*, respectively, and are elevated in the livers of miR-378/378* KO mice. Consistent with these targets as contributors to the metabolic actions of miR-378 and miR-378*, previous studies have implicated carnitine O-acetyltransferase and MED13 in metabolic syndrome and obesity. Our findings identify miR-378 and miR-378* as integral components of a regulatory circuit that functions under conditions of metabolic stress to control systemic energy homeostasis and the overall oxidative capacity of insulin target tissues. Thus, these miRNAs provide potential targets for pharmacologic intervention in obesity and metabolic syndrome.
- Soil and Site Characterization Using Electromagnetic WavesLiu, Ning (Virginia Tech, 2007-03-27)Success in geotechnical analysis, design, and construction invariably requires that we have proper knowledge and understanding of (1) the strength, (2) the fluid flow properties, and (3) the stress-deformation behavior of the earth materials. These important engineering properties are primarily determined by the components and structure of a soil, which also dictate the soil's responses in an electromagnetic field. As a nondestructive technique, the electromagnetic property measurement offers a promising approach to characterize earth materials and identify the effects of changes in environments. However, despite many investigations in the last several decades, the relationship between the frequency-dependent electromagnetic properties of soils and their components and structure are still not well understood. Hence, estimation of engineering properties of a soil in a quantitative way from electromagnetic measurements can be uncertain. In this research several tasks have been accomplished: (1) Development of a physically based model that provides a means of investigating the coupled effects of important polarization mechanisms on soil electromagnetic properties, and a means of relating the electromagnetic properties of a soil to its fines content, clay mineralogy, anisotropy, degree of flocculation and pore fluid chemistry; (2) Proposal of a practically applicable method to determine the volumetric water content, specific surface area and pore fluid salt concentration simultaneously from the dielectric spectrum; (3) Deduction of the wide-frequency electromagnetic properties of a soil by measuring its responses to a step pulse voltage using time domain reflectometry (TDR); (4) Establishment of the relationships between the specific surface area and compressibility, residual shear strength and hydraulic conductivity. This study establishes a framework for quantifying soil engineering properties from their electromagnetic properties. If properly determined and interpreted, the electromagnetic properties can also provide insights into the causes of soil property changes over time and can be very useful in studying the effects of biological factors in geotechnical engineering, a field that may offer great potential for future advances.