Browsing by Author "Liu, Yi-Ping"
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- Expression Profiles of Cellular Retinol-binding Protein, Type II (CRBP II) in Erlang Mountainous ChickensYin, H. D.; Tian, K.; Li, D. Y.; Gilbert, Elizabeth R.; Xiao, L. H.; Chen, S. Y.; Wang, Y.; Liu, Yi-Ping; Zhao, X. L.; Zhu, Q. (2014-03)Cellular retinol-binding protein II (CRBP II) belongs to the family of cellular retinol-binding proteins and plays a major role in absorption, transport, and metabolism of vitamin A. In addition, because vitamin A is correlated with reproductive performance, we measured CRBP II mRNA abundance in erlang mountainous chickens by real-time PCR using the relative quantification method. The expression of CRBP II showed a tissue-specific pattern and egg production rate-dependent changes. The expression was very high (p<0.05) in jejunum and liver, intermediate in kidney, ovary, and oviduct, and lowest (p<0.05) in heart, hypothalamus, and pituitary. In the hypothalamus, oviduct, ovary, and pituitary, CRBP II mRNA abundance were correlated to egg production rate, which increased from 12 wk to 32 wk, peaked at 32 wk relative to the other time points, and then decreased from 32 wk to 45 wk. In contrast, the expression of CRBP II mRNA in heart, jejunum, kidney, and liver was not different at any of the ages evaluated in this study. These data may help to understand the genetic basis of vitamin A metabolism, and suggest that CRBP II may be a candidate gene to affect egg production traits in chickens.
- MUSTN1 mRNA Abundance and Protein Localization is Greatest in Muscle Tissues of Chinese Meat-Quality ChickensLi, Juan; Chen, Yang; Wang, Ya-Gang; Zhao, Xiaoling; Gilbert, Elizabeth R.; Liu, Yi-Ping; Wang, Yan; Hu, Yao-Dong; Zhu, Qing (MDPI, 2013-03-08)The Mustang, Musculoskeletal Temporally Activated Novel-1 Gene (MUSTN1) plays an important role in regulating musculoskeletal development in mammals. We evaluated the developmental and tissue-specific regulation of MUSTN1 mRNA and protein abundance in Erlang Mountainous (EM) chickens. Results indicated that MUSTN1 mRNA/protein was expressed in most tissues with especially high expression in heart and skeletal muscle. The MUSTN1 protein localized to the nucleus in myocardium and skeletal muscle fibers. There were significant differences in mRNA and protein abundance among tissues, ages and between males and females. In conclusion, MUSTN1 was expressed the greatest in skeletal muscle where it localized to the nucleus. Thus, in chickens MUSTN1 may play a vital role in muscle development.
- Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type ChickensZhang, Lu; Zhu, Qing; Liu, Yi-Ping; Gilbert, Elizabeth R.; Li, Diyan; Yin, Huadong; Wang, Yan; Yang, Zhiqin; Wang, Zhen; Yuan, Yuncong; Zhao, Xiaoling (Asian-Australasian Assoc Animal Production Soc, 2015-06-01)