Browsing by Author "Lu, Ming"
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- The Development of High-Throughput and Miniaturized Differential Scanning Calorimeter for Thermodynamic Study of Bio-MoleculesYu, Shifeng (Virginia Tech, 2019-02-19)Biomolecular interactions are fundamentally important for a wide variety of biological processes. Understanding the temperature dependence of biomolecular interactions is hence critical for applications in fundamental sciences and drug discovery. Micro-Electro-Mechanical Systems (MEMS) technology holds great potential in facilitating temperature-dependent characterization of biomolecular interactions by providing on-chip microfluidic handling with drastically reduced sample consumption, and well controlled micro- or nanoscale environments in which biomolecules are effectively and efficiently manipulated and analyzed. This dissertation is focused on a high-through and miniaturized differential scanning calorimeter for thermodynamic study of bio-molecules using MEMS techniques. The dissertation firstly introduces the overall design and operation principles. This miniaturized DSC was fabricated based on a polyimide (PI) thin film. Highly temperature sensitive vanadium oxide was used as the thermistor material. A PDMS (Polydimethylsiloxane) microfluidic chamber was separately fabricated and then bonded firmly with the PI substrate by a stamp-and-stick method. Meanwhile, the micro heater design was optimized to reach better uniformity. A heating stage was constructed for fast and reliable scanning. In this study, we used syringes to deliver the 0.63 μL liquid sample into both the sample and reference chambers. All the testing processes were functionalized using the LabVIEW programs. The sensing material was also characterized. To seek a higher temperature coefficient of resistance (TCR) and less resistive behavior, explorations about various PVD (physical vapor deposition) parameters and annealing conditions were conducted for optimization. In this research, we found vanadium oxide deposited under certain conditions leads to the highest TCR value (a maximum of 2.51%/oC). To better understand the material’s property, we also did the XRD (X-ray Diffraction), SEM (Scanning electron microscope). The micro calorimeter was calibrated using a step thermal response. The time constant was around 3s, the thermal conductance was 0.6mW/K, and the sensitivity was 6.1V/W. The static power resolution of the device at equilibrium is 100 nW, corresponding to 250 nJ/K. These performances confirmed the design and material to be appropriate for both good thermal isolation and power sensitivity. We demonstrated the miniaturized DSC’s performance on several different kinds of protein samples: lysozyme, and mAb (monoclonal antibody) and a DVD IgG (double variable domain immunoglobulin G). The results were found to be reasonable by comparing it with the commercial DSC’s tests. Finally, this instrument may be ideal for incorporation into high throughput screening workflows for the relative comparison of thermal properties between large numbers of proteins when only small quantities are available. The micro-DSC has the potential to characterize the thermal stability of the protein sample with significantly higher throughput and less sample consumption, which could potentially reduce the time and cost for the drug formulation in the pharmaceutical industry.
- A High-Throughput MEMS-Based Differential Scanning Calorimeter for Direct Thermal Characterization of AntibodiesYu, Shifeng; Wu, Yongjia; Wang, Shuyu; Siedler, Michael; Ihnat, Peter M.; Filoti, Dana I.; Lu, Ming; Zuo, Lei (MDPI, 2022-06-16)Calorimeters, which can be used for rapid thermal characterization of biomolecules, are getting intense attention in drug development. This paper presents a novel MEMS-based differential scanning calorimeter (DSC) for direct thermal characterization of protein samples. The DSC consisted of a pair of temperature sensors made by vanadium oxide (VOx) film with a temperature coefficient of resistivity of −0.025/K at 300 K, a microfluidic device with high thermal insulation (2.8 K/mW), and a Peltier heater for linear temperature scanning. The DSC exhibited high sensitivity (6.1 µV/µW), low noise (0.4 µW), high scanning rate (45 K/min), and low sample consumption volume (0.63 µL). The MEMS DSC was verified by measuring the temperature-induced denaturation of lysozyme at different pH, and then used to study the thermal stability of a monoclonal antibody (mAb), an antigen-binding fragment (Fab), and a dual variable domain immunoglobulin (DVD-Ig) at pH = 6. The results showed that lysozyme is a stable protein in the pH range of 4.0–8.0. The protein stability study revealed that the transition temperatures of the intact Fab fragment, mAb, and DVD proteins were comparable with conformational stability results obtained using conventional commercial DSC. These studies demonstrated that the MEMS DSC is an effective tool for directly understanding the thermal stability of antibodies in a high-throughput and low-cost manner compared to conventional calorimeters.
- Review of MEMS differential scanning calorimetry for biomolecular studyYu, Shifeng; Wang, Shuyu; Lu, Ming; Zuo, Lei (2017-12)Differential scanning calorimetry (DSC) is one of the few techniques that allow direct determination of enthalpy values for binding reactions and conformational transitions in biomolecules. It provides the thermodynamics information of the biomolecules which consists of Gibbs free energy, enthalpy and entropy in a straightforward manner that enables deep understanding of the structure function relationship in biomolecules such as the folding/unfolding of protein and DNA, and ligand bindings. This review provides an up to date overview of the applications of DSC in biomolecular study such as the bovine serum albumin denaturation study, the relationship between the melting point of lysozyme and the scanning rate. We also introduce the recent advances of the development of micro-electro-mechanic-system (MEMS) based DSCs.
- Synergetic Attenuation of Stray Magnetic Field in Inductive Power TransferLu, Ming (Virginia Tech, 2017-07-28)Significant stray magnetic field exists around the coils when charging the electric vehicles (EVs) with inductive power transfer (IPT), owning to the large air gap between the transmitter and receiver. The methods for field attenuation usually introduce extra losses and reduce the efficiency. This study focuses on the synergetic attenuation of stray magnetic field which is optimized simultaneously with the efficiency. The optimization is realized with Pareto front. In this dissertation, three methods are discussed for the field attenuation. The first method is to tune the physical parameters of the winding, such as the inner radii, outer radii, distribution of the turns, and types of the litz wires. The second method is to add metal shields around the IPT coils, in which litz wires are used as shields to reduce the shielding losses. The third method is to control the phases of winding currents, which avoids increasing the size and weight of the IPT coils. To attenuate the stray magnetic field by tuning the physical parameters, the conventional method is to sweep all the physical parameters in finite-element simulation. This takes thousands of simulations to derive the Pareto front, and it's especially time-consuming for three-dimensional simulations. This dissertation demonstrates a faster method to derive the Pareto front. The windings are replaced by the lumped loops. As long as the number of turns for each loop is known, the efficiency and magnetic field are calculated directly from the permeance matrices and current-to-field matrices. The sweep of physical parameters in finite-element simulation is replaced by the sweep of the turns numbers for the lumped loops in calculation. Only tens of simulations are required in the entire procedure, which are used to derive the matrices. An exemplary set of coils was built and tested. The efficiency from the matrix calculation is the same as the experimental measurement. The difference for stray magnetic field is less than 12.5%. Metal shields attenuate the stray magnetic field effectively, but generates significant losses owning to the uneven distribution of shield currents. This dissertation uses litz wires to replace the conventional plate shield or ring shield. Skin effect is eliminated so the shield currents are uniformly distributed and the losses are reduced. The litz shields are categorized to two types: shorted litz shield and driven litz shield. Circuit models are derived to analyze their behaviors. The concept of lumped-loop model is applied to derive the Pareto front of efficiency versus stray magnetic field for the coils with litz shield. In an exemplary IPT system, coils without metal shield and with metal shields are optimized for the same efficiency. Both the simulation and experimental measurement verify that the shorted litz shield has the best performance. The stray magnetic field is attenuated by 65% compared to the coils without shield. This dissertation also introduces the method to attenuate the stray magnetic field by controlling the phases of winding currents. The magnetic field around the coils is decomposed to the component in the axial direction and the component in the radial direction. The axial component decreases with smaller phase difference between windings' currents, while the radial component exhibits the opposite property. Because the axial component is dominant around the IPT coils, decreasing the phase difference is preferred. The dual-side-controlled converter is applied for the circuit realization. Bridges with active switches are used for both the inverter on the transmitter side and the rectifier on the receiver side. The effectiveness of this method was verified both in simulation and experiment. Compared to the conventional series-series IPT with 90° phase difference between winding currents, stray magnetic field was attenuated by up to 30% and 40% when the phase differences of winding currents are 50° and 40°, respectively. Furthermore, an analytical method is investigated to calculate the proximity-effect resistance of the planar coils with ferrite plate. The objective of this method is to work together with the fast optimization which uses the lumped-loop model. The existence of the ferrite plate complicates the calculation of the magnetic field across each turn which is critical to derive the proximity-effect resistance. In this dissertation, the ferrite plate is replaced by the mirrored turns according to the method of image. The magnetic fields are then obtained from Ampere's Law and Biot-Savart Law. Up to 200 kHz, the difference of the proximity-effect resistance is less than 15% between calculation and measurement.
- System Dynamics Model for Testing and Evaluating Automatic Headway Control Models for Trucks Operating on Rural HighwaysLu, Ming (Virginia Tech, 1996-04-01)The objective of this research is to explore a methodology that can be used for testing and evaluating AVCS technologies, and, in particular, automatic headway control models for trucks operating on rural highways. The emphasis is put on the realization of vehicle headway control in the real world highway systems. System dynamics has been selected as the simulation tool for developing, testing and evaluating vehicle headway control models. The following behavior of human driver in a real world highway environment is studied and simulated. An automatic headway control model, Multiple-mode Vehicle Headway Control (MVHC) model, is developed for single lane, cars and trucks mixed flow control in a rural highway system. Using safety and motorist comfort as MOE criteria and the acceleration noise as the index of motorist comfort, some selected automatic headway control models are evaluated. This study demonstrated that simulation affords a means of modeling control processes with various certain and uncertain factors, and therefore, it plays a key role in the development of automatic headway control systems.
- Thermoelectric properties of DC-sputtered filled skutterudite thin filmFu, Gaosheng; Zuo, Lei; Chen, Jie; Lu, Ming; Yu, Liangyao (American Institute of Physics, 2015-03-28)The Yb filled CoSb3 skutterudite thermoelectric thin films were prepared by DC magnetron sputtering. The electrical conductivity, Seebeck coefficient, thermal conductivity, and figure of merit ZT of the samples are characterized in a temperature range of 300K to 700 K. X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy are obtained to assess the phase composition and crystallinity of thin film samples at different heat treatment temperatures. Carrier concentrations and Hall mobilities are obtained from Hall Effect measurements, which provide further insight into the electrical conductivity and Seebeck coefficient mechanisms. The thermal conductivity of thin film filled skutterudite was found to be much less compared with bulk Yb filled CoSb3 skutterudite. In this work, the 1020K heat treatment was adopted for thin film post process due to the high degree of crystallinity as well as avoiding reverse heating effect. Thin film samples of different thicknesses were prepared with the same sputtering deposition rate and maximum ZT of 0.48 was achieved at 700K for the 130 nm thick sample. This value was between half and one third of the bulk figure of merit which was due to the lower Hall mobility. (C) 2015 AIP Publishing LLC.