Browsing by Author "Mosig, Rebecca A."
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- Natural antisense transcript of Period2, Per2AS, regulates the amplitude of the mouse circadian clockMosig, Rebecca A.; Castaneda, Allison N.; Deslauriers, Jacob C.; Frazier, Landon P.; He, Kevin L.; Maghzian, Naseem; Pokharel, Aarati; Schrier, Camille T.; Zhu, Lily; Koike, Nobuya; Tyson, John J.; Green, Carla B.; Takahashi, Joseph S.; Kojima, Shihoko (Cold Spring Harbor Laboratory Press, 2021-05-20)In mammals, a set of core clock genes form transcription–translation feedback loops to generate circadian oscillations. In mammals, a set of core clock genes form transcription–translation feedback loops to generate circadian oscillations. We and others recently identified a novel transcript at the Period2 (Per2) locus that is transcribed from the antisense strand of Per2. This transcript, Per2AS, is expressed rhythmically and antiphasic to Per2 mRNA, leading to our hypothesis that Per2AS and Per2 mutually inhibit each other’s expression and form a double negative feedback loop. By perturbing the expression of Per2AS,we found that Per2AS transcription, but not transcript, represses Per2. However, Per2 does not repress Per2AS, as Per2 knockdown led to a decrease in the Per2AS level, indicating that Per2AS forms a single negative feedback loop with Per2 and maintains the level of Per2 within the oscillatory range. Per2AS also regulates the amplitude of the circadian clock, and this function cannot be solely explained through its interaction with Per2, as Per2 knockdown does not recapitulate the phenotypes of Per2AS perturbation. Overall, our data indicate that Per2AS is an important regulatory molecule in the mammalian circadian clock machinery. Our work also supports the idea that antisense transcripts of core clock genes constitute a common feature of circadian clocks, as they are found in other organisms.
- Timing without coding: How do long non-coding RNAs regulate circadian rhythms?Mosig, Rebecca A.; Kojima, Shihoko (Academic Press-Elsevier, 2022-06-01)Long non-coding RNAs (lncRNAs) are a new class of regulatory RNAs that play important roles in disease development and a variety of biological processes. Recent studies have underscored the importance of lncRNAs in the circadian clock system and demonstrated that lncRNAs regulate core clock genes and the core clock machinery in mammals. In this review, we provide an overview of our current understanding of how lncRNAs regulate the circadian clock without coding a protein. We also offer additional insights into the challenges in understanding the functions of lncRNAs and other unresolved questions in the field. We do not cover other regulatory ncRNAs even though they also play important roles; readers are highly encouraged to refer to other excellent reviews on this topic.