Browsing by Author "Nguyen, Brian"
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- CS3604 Case Study LibraryWieder, Brian; Papili, Kyle; Jain, Sohil; Bekele, Kidus; Marin, Drew; Nguyen, Brian (Virginia Tech, 2022-05-07)For this project, our job was to create a website to house the previous years’ case study presentations. To do this we needed to support all of the file types that could be submitted such as PowerPoint, a PDF document, an MP4 video, and a Google Slides link. These files also needed to be categorized to allow for searching based on a variety of categories. These categories include the date, topic, keywords, file format, etc. These files would form a repository of case studies for future CS3604 students to look at as they create their case study presentations. Students can build off these cases or create their own. Students then would submit their case studies directly to the website. To accomplish this we used AWS to deploy the website directly onto the Virginia Tech Digital Library Platform. The custom domain for the website can be found at casestudies.cs.vt.edu. The CS department has funded an AWS account for the website and 638 presentation files have been uploaded to the website. As a team, we tested the website to ensure that a specific presentation could be searched for and that the file was able to be accessed by the user.
- Integrated proteomic and transcriptomic analysis of the Aedes aegypti eggshellMarinotti, Osvaldo; Ngo, Tuan; Kojin, Bianca B.; Chou, Shao-Pei; Nguyen, Brian; Juhn, Jennifer; Carballar-Lejarazú, Rebeca; Marinotti, Pedro N.; Jiang, Xiaofang; Walter, Marika F.; Tu, Zhijian Jake; Gershon, Paul D.; James, Anthony A. (2014-04-05)Background Mosquito eggshells show remarkable diversity in physical properties and structure consistent with adaptations to the wide variety of environments exploited by these insects. We applied proteomic, transcriptomic, and hybridization in situ techniques to identify gene products and pathways that participate in the assembly of the Aedes aegypti eggshell. Aedes aegypti population density is low during cold and dry seasons and increases immediately after rainfall. The survival of embryos through unfavorable periods is a key factor in the persistence of their populations. The work described here supports integrated vector control approaches that target eggshell formation and result in Ae. aegypti drought-intolerant phenotypes for public health initiatives directed to reduce mosquito-borne diseases. Results A total of 130 proteins were identified from the combined mass spectrometric analyses of eggshell preparations. Conclusions Classification of proteins according to their known and putative functions revealed the complexity of the eggshell structure. Three novel Ae. aegypti vitelline membrane proteins were discovered. Odorant-binding and cysteine-rich proteins that may be structural components of the eggshell were identified. Enzymes with peroxidase, laccase and phenoloxidase activities also were identified, and their likely involvements in cross-linking reactions that stabilize the eggshell structure are discussed.