Browsing by Author "Nickerson, Stephen C."
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- Artificial Induction of Lactation in Nonbreeder Dairy CowsJewell, Tracy Michelle (Virginia Tech, 2002-08-09)Thirty-four cows (26 Holsteins and 8 Jerseys) were subjected to an estrous synchronization protocol administering 2 PGF2Æ Ã injections 11 d apart prior to beginning the lactation-induction protocol. Artificial induction of lactation yielded a 92% success rate for Holstein cows with success defined as achieving >9 kg milk/d, and a 88% success rate for Jersey cows with success defined as achieving > 5 kg milk/d. Mean accumulated milk yield for induced cows at 150 DIM was 65% of mean yield for nontreated cows. Mean peak milk yield for lactation- induced Holsteins and Jerseys was 32 kg/d and 20 kg/d, respectively. Mean serum and milk progesterone concentrations for samples collected during the first 6 d of lactation were not different between lactation-induced and nontreated cows. However, mean serum estradiol concentrations for induced cows were higher (P <0.05) in samples collected 3 and 5 DIM. Lactation-induced cows exhibited an increase in serum alpha-lactalbumin concentrations 2 d prior to initiation of milking, reaching values of ~260 ng/ml. Mean days-to-first service was greatly reduced in cows induced into lactation compared to nontreated cows, while mean services per conception was similar between induced and nontreated cows. Mean days to conception was lower for induced cows than for nontreated cows. By 150 DIM, pregnancy rate of induced cows was 70%, whereas nontreated cows averaged 56% pregnancy rate.
- Curli Production and ERIC-PCR Fingerprinting of Escherichia coli from Clinical Cases of Bovine MastitisDyer, John Gilbert (Virginia Tech, 2005-02-04)Mastitis caused by Escherichia coli is among the most common and severe forms of environmental mastitis. Currently, the severity of E. coli mastitis is thought to be more related to cow factors than bacterial virulence. Some strains of E. coli, however, may be adapted to colonizing mammary tissue, increasing clinical severity, and impairing recovery. Curli are adhesive surface structures produced by some E. coli and Salmonella strains that bind a number of host proteins and have recently been found to play a role in the pathogenesis of bacterial sepsis. Sixty-one E. coli isolates from 36 clinical cases of bovine mastitis were characterized using ERIC-PCR and screened for their ability to produce curli by binding Congo-red dye. The effect of curli production on case recovery, based on a return to milk production, was investigated for a subset of 43 isolates from 20 quarters of 19 cows. Fifty-eight of the 61 isolates were clustered into two clonal groups at 52% genetic similarity. Thirty-five of all 61 isolates (57%) were curli-positive. Twenty-three isolates from 13 cows clustered in clonal group I, of which 5 cases (38%) were curli-positive; 35 isolates from 22 cows were clustered in clonal group II, 15 of which were curli-positive cases (68%). No association was found between genetic similarity and phenotypic curli expression of isolates from cows with clinical E. coli mastitis cases (p=0.16). Phenotypic curli expression in isolates did not affect the recovery of cows' milk yield to pre-mastitis production levels (p=0.18).
- The Effect of Maternal and Fetal Inbreeding on Dystocia, Calf Survival, Days to First Service and Non-Return Performance in U.S. Dairy CattleAdamec, Vaclav (Virginia Tech, 2002-01-07)Intensive selection for increased milk production over many generations has led to growing genetic similarity and increased relationships in dairy population. In the current study, inbreeding depression was estimated for number of days to first service, summit milk, conception by 70 days non-return, and calving rate with a linear mixed model (LMM) approach and for calving difficulty, calf mortality with a Bayesian threshold model (BTM) for categorical traits. Effectiveness of classical and unknown parentage group procedures to estimate inbreeding coefficients was evaluated depending on completeness of a 5-generation pedigree. A novel method derived from the classical formula to estimate inbreeding was utilized to evaluate completeness of pedigrees. Two different estimates of maternal inbreeding were fitted in separate models as a linear covariate in combined LMM analyses (Holstein registered and grade cows and Jersey cows) or separate analyses (registered Holstein cows) by parity (1-4) with fetal inbreeding. Impact of inbreeding type, model, data structure, and treatment of herd-year-season (HYS) on magnitude and size of inbreeding depression were assessed. Grade Holstein datasets were sampled and analyzed by percentage of pedigree present (0-30%, 30-70% and 70-100%). BTM analyses (sire-mgs) were performed using Gibbs sampling for parities 1, 2 and 3 fitting maternal inbreeding only. In LMM analyses of grade data, the least pedigree and diagonal A matrix performed the worst. Significant inbreeding effects were obtained in most traits in cows of parity 1. Fetal inbreeding depression was mostly lower than that from maternal inbreeding. Inbreeding depression in binary traits was the most difficult to evaluate. Analyses with non-additive effects included in LMM, for data by inbreeding level and by age group should be preferred to estimate inbreeding depression. In BTM inbreeding effects were strongly related to dam parity and calf sex. Largest effects were obtained from parity 1 cows giving birth to male calves (0.417% and 0.252% for dystocia and calf mortality) and then births to female calves (0.300% and 0.203% for dystocia and calf mortality). Female calves from mature cows were the least affected (0.131% and 0.005% for dystocia and calf mortality). Data structure was found to be a very important factor to attainment of convergence in distribution.
- Estimation of the Economic Impact of a Unit Change in Predicted Transmitting Ability for Daughter Pregnancy Rate and Other Predicted Transmitting Ability in the Merit IndexesYook, Eunsun (Virginia Tech, 2004-04-28)These studies deal with lifetime profit analyses for individual cows, and using these estimates to determine the economic value of genetic changes in traits for which genetic evaluations (predicted transmitting ability, PTA) are currently available. Data were collected from six states processed by Dairy Records Management Systems (DRMS) from herds on continuous test for at least 10 yr. The purpose of the first study was to determine how well estimators of lifetime net income based on 305-d lactation yields and a 10-yr opportunity (RNI305.10) and based on complete lactation data but a 5-yr opportunity (RNIc.5) predict the estimate based on complete lactations and a 10-yr opportunity (RNIc.10). Records for 22,854 cows in Virginia herds born in 1988, 1990, and 1992 from the DRMS in Raleigh, NC were used. Each RNI was calculated using fluid (skim/fat) pricing and milk-fat-protein pricing. Regression analyses including herd and birth year were used in the model to estimate the regression of RNIc.10 on RNIc.5, and RNIc.10 on RNI305.10. The resulting regression coefficients for fluid (skim/fat) pricing were $1.53 and $1.12 explaining 67 and 97% of the variation of RNIc.10, respectively. The corresponding results for milk-fat-protein pricing were $1.52 and $1.14 explaining 68 and 96% of the variation of RNIc.10, respectively. Using RNIc.10 as the measure to estimate lifetime profit is strongly recommended over the two alternatives tested. In the second study, the economic impacts of a unit change in PTA of daughter pregnancy rate (DPR) and other PTA in the merit indexes on lifetime profit estimates of a bull's daughters were estimated to determine an economic weight for the PTADPR and other PTA in economic indexes. Records for 71,094 cows born in 1988, 1990, and 1992 from six states processed at DRMS were used: Florida [10,940 cows], Indiana [8,231 cows], North Carolina [12,280 cows], Texas [4,786 cows], Virginia [20,341 cows], and Vermont [14,516 cows]. The basic RNI function consisted of [total milk, fat, and protein income ?feed cost for production] (yield income, YI) + [net value of calves + net salvage value] (non yield income, NYI) ?rearing cost (RC) ?[(daily cost for labor, maintenance feed, supplies, and fixed expenses) x days in herd] (daily cost, DC). Some of the economic impacts of PTA described for the merit indexes were not included in the basic RNI. These were added to RNI by multiplying the respective sire PTA by the economic impact. These included -165*PTASCS (M); 33*udder composite + 15*feet and legs composite -14.86*body size composite (T); and 8.064*PTA for daughter pregnancy rate -4.80*PTA for daughter calving ease (PRCE). Each ARNI was calculated using all production records initiated prior to the cow's tenth birthday with three milk pricing systems comparable to the prices in USDA three merit indexes: fluid (skim/fat) pricing (FARNI), milk-fat-protein pricing (NMARNI), and cheese pricing (CARNI). Two levels of prices for rearing cost per day and daily cost were used for calculating FARNI, NMARNI, and CARNI. Regression analyses including herd and birth year in the model were used to estimate the simple and partial regressions of ARNI or partitioned ARNI on sire PTA. Partial regression included all PTA in Net Merit, except service sire calving ease. Ignoring other PTA, one unit increase in PTADPR increased 476.25kg of lifetime total milk or 18 days of total DIM. One unit decrease in PTASCS increased 4372.50kg of lifetime total milk. With low daily and rearing costs, each 1% change in PTADPR increased ARNI by $59.31 to $55.82 depending on the milk pricing systems. The corresponding results with high daily and rearing costs were $27.50 to $24.01. Standardized multiple regression enabled the comparison of the economic weights of this study with those of USDA. The PTA for productive life (PL) in all three USDA merit index was emphasized less than the results from this study; however, PTADPR in USDA indexes was emphasized more than this study. In this study, the economic weight of PTADPR was negative within the low daily and rearing costs, but it was positive in the high daily and rearing costs.
- Intramammary infection in rapidly growing, non-lactating mammary glandsEnger, Benjamin David (Virginia Tech, 2018-08-24)Intramammary infections (IMI) are common in non-lactating heifer and dry cow mammary glands and occur during periods of appreciable mammary growth and development. The presence of these infections is expected to negatively impact mammary growth and development but has yet to be investigated. The works reported here investigated how IMI affects mammary tissue structure, cellularity, and the expression of integral mammogenic hormone receptors implicated in mammary growth. Non-pregnant non-lactating cows (n = 19) were administered estradiol and progesterone to stimulate mammary growth and 2 quarters of each cow were subsequently infused with either saline (n = 19) or Staphylococcus aureus (n = 19). Intramammary infusion of Staphylococcus aureus increased the number of immune cells present in gland secretions and also increased the proportion of neutrophils comprising these secretion somatic cells. Mammary tissues from quarters infused with Staphylococcus aureus contained more immune cells, less mammary epithelial tissue area, and greater tissue areas of intralobular stromal tissue than saline quarters. Staphylococcus aureus quarters also contained more apoptotic mammary epithelial cells and a lower proportion of apoptotic cells in the intralobular stroma compartment than saline infused quarters; this signified that Staphylococcus aureus quarters had less epithelial growth and experienced an expansion and/or lack of regression of stromal tissues. The number of cells expressing estrogen receptor α (ESR1) and progesterone receptor (PGR), as well as staining characteristics of ESR1 and PGR positive nuclei was also examined in these tissues. No appreciable differences were observed in any of the examined ESR1 and PGR measures between Staphylococcus aureus and saline mammary glands, but myoepithelial cells from Staphylococcus aureus glands had a greater nuclear staining area than saline quarters, indicating that these cells were affected by IMI. The results of these investigations indicate that IMI, in mammary glands that are concurrently stimulated to grow and develop, limits the growth of mammary epithelium and impairs regression of the stromal tissue, both of which are necessary for successful lactational performance.
- Nutritional and Management Practices to Reduce Excessive Nutrient Excretion on Dairy FarmsWydner, Fred Preston, III (Virginia Tech, 2003-10-14)A 2-yr field study was conducted to reduce nutrient losses from Virginia dairy farms through nutritional and herd management practices. Ten collaborator herds were identified, all at state DHIA average or better for milk yield and days open. Baseline feed samples and ration information were collected for 2 mo and analyzed for phosphorus (P) and nitrogen (N). Feeds were analyzed monthly, and monthly DHIA milk yield, milk composition, milk urea N (MUN), and reproductive data were recorded. Blood and fecal samples were collected from 25 cows/herd every 3 mo to monitor P excretion and blood urea N. Nutrient balances were developed for each farm for N and P at the start of the study and following ration and management changes. Collaborator herds imported, on average, 290% more N and 320% more P onto the farm than was removed through milk, culled animals, crop sales, or manure sales. By following NRC (1989) recommendations, collaborator farms could reduce N inputs by 21% and P inputs by 45%. Minimizing P in purchased feed, purchased feeds/cow, purchased feeds/ha, and total P input could cause significant reductions in P balance for participating collaborator herds. None of the N variables tested (purchased feed, purchased feed/cow, purchased feed/ha, and total N input) provided significant reductions.
- Staphylococcus aureus intramammary challenge in non-lactating mammary glands stimulated to rapidly grow and develop with estradiol and progesteroneEnger, Benjamin D.; Crutchfield, Carly E.; Yohe, Taylor T.; Enger, Kellie M.; Nickerson, Stephen C.; Parsons, Catherine L. M.; Akers, Robert Michael (2018-06-05)Intramammary infections (IMI) are prevalent in non-lactating dairy cattle and their occurrence during periods of significant mammary growth and development (i.e. pregnant heifers and dry cows) is believed to interfere with growth, development, and subsequent milk production. However, direct study of IMI impacts on non-lactating but developing mammary glands is lacking. The objectives of this study were to (1) define how IMI affected total and differential mammary secretion somatic cell counts in mammary glands stimulated to rapidly grow using estradiol and progesterone, and (2) characterize changes in mammary morphology in response to IMI. Mammary growth was stimulated in 19 non-pregnant, non-lactating cows and 2 quarters of each cow were subsequently infused with either saline (n = 19) or Staphylococcus aureus (n = 19). Mammary secretions were taken daily until mammary tissues were collected at either 5 or 10 days post-challenge. Staph. aureus quarter secretions yielded greater concentrations of somatic cells than saline quarters and contained a greater proportion of neutrophils. Staph. aureus mammary tissues exhibited higher degrees of immune cell infiltration in luminal and intralobular stroma compartments than saline quarters. Infected tissues also contained reduced areas of epithelium and tended to have greater amounts of intralobular stroma. Results indicate that IMI in non-lactating glands that were stimulated to grow, produced immune cell infiltration into mammary tissues and secretions, which was associated with changes in mammary tissue structure. The observed reduction of mammary epithelium indicates that IMI impair mammary development in rapidly growing mammary glands, which may reduce future reduced milk yields.
- Synchronization and Resynchronization of Ovulation and Timed Insemination in Lactating Dairy Cows and HeifersPeeler, Iris Dawn (Virginia Tech, 2004-04-26)This study was conducted to evaluate the efficacy of intravaginal progesterone (P4) inserts (CIDR) in synchronization protocols combined with timed artificial insemination (TAI) as related reproductive performance. In the first study, heifers were synchronized with CIDR inserts followed by TAI. Heifers in the estradiol cypionate (ECP) group were synchronized with a combination of ECP, CIDR, prostaglandin (PGF2α), and ECP (CIDR-ECP), while the gonadotropin-releasing hormone (GnRH) group was synchronized using a combination of ECP, CIDR, PGF2α, and GnRH (CIDR-GnRH). All heifers were bred at either 48, 56, or 72 hours (h) after CIDR removal. Overall pregnancy rate (PR) for synchronized heifers was 60.1%, and embryo survival rate (ESR) was 98%. Pregnancy rate for CIDR-ECP treated heifers was influenced by artificial insemination (AI) time. In conclusion, ECP or GnRH may be used effectively in a CIDR-based TAI program in heifers. In the second study, cows were synchronized with CIDR devices or Ovsynch. The CIDR group received a combination of ECP, CIDR, PGF2α, and GnRH, while the Ovsynch group was synchronized using a combination of GnRH and PGF2α. Cows were bred at either 0, 8, or 24 h after the final GnRH injection. Overall PR for first service was 30.5% with ESR of 82.8%. Overall resynchronization PR was 35.1% with an ESR of 84.8%. In conclusion, Ovsynch and CIDR-based protocols are equally effective in synchronizing ovulation in a TAI program and resulted in comparable PR.