Browsing by Author "Parsons, Martha Anne Cohen"
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- Androgen aromatization and cytosol estradiol-receptors in the mediation of masculine sexual behavior in Japanese quailParsons, Martha Anne Cohen (Virginia Polytechnic Institute and State University, 1982)The neuroendocrinology of masculine sexual behavior was examined in Japanese quail selected bi-directionally for adult mating frequency. Experiments were conducted with brain tissues from functionally castrated, High (HM) and Low Mating (LM) line quail to compare (1) the in vivo aromatization of ³H-testosterone and (2) cytosolic estradiol-binding by in vitro saturation analyses. After in vivo incubation with ³H-testosterone, all radioactivity recovered in brain tissues was in the form of testosterone, dihydrotestosterone, or estradiol. Neither the total ³H nor ³H-testosterone metabolite radioactivity differed upon comparison of the two genetic lines. Of all ³H-testosterone metabolite radioactivity, ³H-estradiol represented 45 ± 6% in the HM line and 46 ± 6% in the LM line, indicating that the line difference in mating frequency was not due to a corresponding difference in aromatase activity. Inasmuch as both the HM and LM line birds actively converted testosterone to estradiol, these results implicated a neural mechanism involving estradiol-receptor interactions. Estradiol-receptor binding parameters were estimated in subsequent experiments by fitting a hyperbolic saturation curve to point measurements of total binding. Using this single-class binding site model, the apparent dissociation constant (Kd) for the estradiol-receptor interaction in the HM line was 0.40 ± .06 nM and the number of specific estradiol binding sites (Bmax) was 10.4±.4 fmoles/mg cytosol protein. For the LM line, an apparent Kd of 0.41 ± .20 nM and a B of 9.50 ± 1.0 fmoles/mg cytosol protein were obtained. The similarity between corresponding binding parameters for the divergent mating lines indicated that the number and/or estradiol-binding affinity of a single class of cytosol receptors was also not responsible for the line difference in mating frequency. Though these results confirm that interaction of estradiol with cytosol receptors was not the limiting neuroendocrinological mechanism in the differential expression of masculine sexual behavior in quail, the possible involvement of dynamic interrelationships between cytoplasmic and nuclear estrogen binding was discussed.
- Protein and sulfur amino acid requirements of male and female breeder turkeysParsons, Martha Anne Cohen (Virginia Polytechnic Institute and State University, 1979)One hundred eight male and 206 female breeder turkeys were fed isocaloric corn-soy rations containing 8, 10 or 12, and 10, 12 or 14'% crude protein, respectively, to which 0.0, 0.1, 0.2, or 0.3% DL-methionine was added in a factorial arrangement of treatments,. With the exception of percentage hen-day egg production and egg size, dietary treatment had no significant effect on the reproduction of females or on the growth of the progeny. Egg production was higher among females fed 12 and 14% protein than among those fed 10% protein. Those fed 14% protein laid the heaviest eggs. Female serum albumin concentrations and the concentrations of blood threonine and lysine significantly increased while concentrations of blood glycine decreased with increased levels of dietary protein. Optimum semen volume and spermatozoa concentration were obtained for males fed 10% protein. For females, the significant response obtained in egg production to increased protein levels and the failure to obtain this response with increased total sulfur amino acid (TSAA) levels as a percentage of total protein, indicates that other amino acids may have been limiting. Based on these results, it appears that for optimum reproduction of Medium White breeder turkeys, a daily intake of at least 22.7 g protein and 696 mg TSAA are necessary for females, and 25.0 g protein and 1,175 mg TSAA are necessary for males.