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Browsing by Author "Richards, Stephen"

Now showing 1 - 4 of 4
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    Analysis of Microsatellite Variation in Drosophila melanogaster with Population-Scale Genome Sequencing
    Fondon, John W. III; Martin, Andy; Richards, Stephen; Gibbs, Richard A.; Mittelman, David (PLoS, 2012-03-12)
    Genome sequencing technologies promise to revolutionize our understanding of genetics, evolution, and disease by making it feasible to survey a broad spectrum of sequence variation on a population scale. However, this potential can only be realized to the extent that methods for extracting and interpreting distinct forms of variation can be established. The error profiles and read length limitations of early versions of next-generation sequencing technologies rendered them ineffective for some sequence variant types, particularly microsatellites and other tandem repeats, and fostered the general misconception that such variants are inherently inaccessible to these platforms. At the same time, tandem repeats have emerged as important sources of functional variation. Tandem repeats are often located in and around genes, and frequent mutations in their lengths exert quantitative effects on gene function and phenotype, rapidly degrading linkage disequilibrium between markers and traits. Sensitive identification of these variants in large-scale next-gen sequencing efforts will enable more comprehensive association studies capable of revealing previously invisible associations. We present a population-scale analysis of microsatellite repeats using whole-genome data from 158 inbred isolates from the Drosophila Genetics Reference Panel, a collection of over 200 extensively phenotypically characterized isolates from a single natural population, to uncover processes underlying repeat mutation and to enable associations with behavioral, morphological, and life-history traits. Analysis of repeat variation from next-generation sequence data will also enhance studies of genome stability and neurodegenerative diseases.
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    Brown marmorated stink bug, Halyomorpha halys (Stål), genome: putative underpinnings of polyphagy, insecticide resistance potential and biology of a top worldwide pest
    Sparks, Michael E.; Bansal, Raman; Benoit, Joshua B.; Blackburn, Michael B.; Chao, Hsu; Chen, Mengyao; Cheng, Sammy; Childers, Christopher; Dinh, Huyen; Doddapaneni, Harsha V.; Dugan, Shannon; Elpidina, Elena N.; Farrow, David W.; Friedrich, Markus; Gibbs, Richard A.; Hall, Brantley; Han, Yi; Hardy, Richard W.; Holmes, Christopher J.; Hughes, Daniel S. T.; Ioannidis, Panagiotis; Cheatle Jarvela, Alys M.; Johnston, J. Spencer; Jones, Jeffery W.; Kronmiller, Brent A.; Kung, Faith; Lee, Sandra L.; Martynov, Alexander G.; Masterson, Patrick; Maumus, Florian; Munoz-Torres, Monica; Murali, Shwetha C.; Murphy, Terence D.; Muzny, Donna M.; Nelson, David R.; Oppert, Brenda; Panfilio, Kristen A.; Paula, Débora P.; Pick, Leslie; Poelchau, Monica F.; Qu, Jiaxin; Reding, Katie; Rhoades, Joshua H.; Rhodes, Adelaide; Richards, Stephen; Richter, Rose; Robertson, Hugh M.; Rosendale, Andrew J.; Tu, Zhijian Jake; Velamuri, Arun S.; Waterhouse, Robert M.; Weirauch, Matthew T.; Wells, Jackson T.; Werren, John H.; Worley, Kim C.; Zdobnov, Evgeny M.; Gundersen-Rindal, Dawn E. (2020-03-14)
    Background Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This species is also a nuisance due to overwintering in human-made structures. It has caused significant agricultural losses in recent years along the Atlantic seaboard of North America and in continental Europe. Genomic resources will assist with determining the molecular basis for this species’ feeding and habitat traits, defining potential targets for pest management strategies. Results Analysis of the 1.15-Gb draft genome assembly has identified a wide variety of genetic elements underpinning the biological characteristics of this formidable pest species, encompassing the roles of sensory functions, digestion, immunity, detoxification and development, all of which likely support H. halys’ capacity for invasiveness. Many of the genes identified herein have potential for biomolecular pesticide applications. Conclusions Availability of the H. halys genome sequence will be useful for the development of environmentally friendly biomolecular pesticides to be applied in concert with more traditional, synthetic chemical-based controls.
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    Genomic analysis of two phlebotomine sand fly vectors of leishmania from the new and old World
    Labbe, Frederic; Abdeladhim, Maha; Abrudan, Jenica; Araki, Alejandra Saori; Araujo, Ricardo N.; Arensburger, Peter; Benoit, Joshua B.; Brazil, Reginaldo Pecanha; Bruno, Rafaela V.; Rivas, Gustavo Bueno da Silva D. S.; de Abreu, Vinicius Carvalho; Charamis, Jason; Coutinho-Abreu, Iliano V.; da Costa-Latge, Samara G.; Darby, Alistair; Dillon, Viv M.; Emrich, Scott J.; Fernandez-Medina, Daniela; Gontijo, Nelder Figueiredo; Flanley, Catherine M.; Gatherer, Derek; Genta, Fernando A.; Gesing, Sandra; Giraldo-Calderon, Gloria I.; Gomes, Bruno; Aguiar, Eric Roberto Guimaraes Rocha; Hamilton, James GC C.; Hamarsheh, Omar; Hawksworth, Mallory; Hendershot, Jacob M.; Hickner, Paul V.; Imler, Jean-Luc; Ioannidis, Panagiotis; Jennings, Emily C.; Kamhawi, Shaden; Karageorgiou, Charikleia; Kennedy, Ryan C.; Krueger, Andreas; Latorre-Estivalis, Jose M.; Ligoxygakis, Petros; Meireles-Filho, Antonio Carlos A.; Minx, Patrick; Miranda, Jose Carlos; Montague, Michael J.; Nowling, Ronald J.; Oliveira, Fabiano; Ortigao-Farias, Joao; Pavan, Marcio G.; Pereira, Marcos Horacio; Pitaluga, Andre Nobrega; Olmo, Roenick Proveti; Ramalho-Ortigao, Marcelo; Ribeiro, Jose MC C.; Rosendale, Andrew J.; Sant'Anna, Mauricio RV V.; Scherer, Steven E.; Secundino, Nagila FC C.; Shoue, Douglas A.; Moraes, Caroline da Silva D. S.; Gesto, Joao Silveira Moledo; Souza, Nataly Araujo; Syed, Zainulabueddin; Tadros, Samuel; Teles-de-Freitas, Rayane; Telleria, Erich L.; Tomlinson, Chad; Traub-Cseko, Yara M.; Marques, Joao Trindade; Tu, Zhijian; Unger, Maria F.; Valenzuela, Jesus; Ferreira, Flavia; de Oliveira, Karla PV V.; Vigoder, Felipe M.; Vontas, John; Wang, Lihui; Weedall, Gareth D.; Zhioua, Elyes; Richards, Stephen; Warren, Wesley C.; Waterhouse, Robert M.; Dillon, Rod J.; McDowell, Mary Ann (Public Library of Science, 2023-04-12)
    Phlebotomine sand flies are of global significance as important vectors of human disease, transmitting bacterial, viral, and protozoan pathogens, including the kinetoplastid parasites of the genus Leishmania, the causative agents of devastating diseases collectively termed leishmaniasis. More than 40 pathogenic Leishmania species are transmitted to humans by approximately 35 sand fly species in 98 countries with hundreds of millions of people at risk around the world. No approved efficacious vaccine exists for leishmaniasis and available therapeutic drugs are either toxic and/or expensive, or the parasites are becoming resistant to the more recently developed drugs. Therefore, sand fly and/or reservoir control are currently the most effective strategies to break transmission. To better understand the biology of sand flies, including the mechanisms involved in their vectorial capacity, insecticide resistance, and population structures we sequenced the genomes of two geographically widespread and important sand fly vector species: Phlebotomus papatasi, a vector of Leishmania parasites that cause cutaneous leishmaniasis, (distributed in Europe, the Middle East and North Africa) and Lutzomyia longipalpis, a vector of Leishmania parasites that cause visceral leishmaniasis (distributed across Central and South America). We categorized and curated genes involved in processes important to their roles as disease vectors, including chemosensation, blood feeding, circadian rhythm, immunity, and detoxification, as well as mobile genetic elements. We also defined gene orthology and observed micro-synteny among the genomes. Finally, we present the genetic diversity and population structure of these species in their respective geographical areas. These genomes will be a foundation on which to base future efforts to prevent vector-borne transmission of Leishmania parasites.
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    Unique features of a global human ectoparasite identified through sequencing of the bed bug genome
    Benoit, Joshua B.; Adelman, Zach N.; Reinhardt, Klaus; Dolan, Amanda M.; Poelchau, Monica; Jennings, Emily C.; Szuter, Elise M.; Hagan, Richard W.; Gujar, Hemant; Shukla, Jayendra Nath; Zhu, Fang; Mohan, M.; Nelson, David R.; Rosendale, Andrew J.; Derst, Christian; Resnik, Valentina; Wernig, Sebastian; Menegazzi, Pamela; Wegener, Christian; Peschel, Nicolai; Hendershot, Jacob M.; Blenau, Wolfgang; Predel, Reinhard; Johnston, Paul R.; Ioannidis, Panagiotis; Waterhouse, Robert M.; Nauen, Ralf; Schorn, Corinna; Ott, Mark-Christoph; Maiwald, Frank; Johnston, J. Spencer; Gondhalekar, Ameya D.; Scharf, Michael E.; Peterson, Brittany F.; Raje, Kapil R.; Hottel, Benjamin A.; Armisen, David; Crumiere, Antonin Jean Johan; Refki, Peter Nagui; Santos, Maria Emilia; Sghaier, Essia; Viala, Severine; Khila, Abderrahman; Ahn, Seung-Joon; Childers, Christopher; Lee, Chien-Yueh; Lin, Han; Hughes, Daniel S. T.; Duncan, Elizabeth J.; Murali, Shwetha C.; Qu, Jiaxin; Dugan, Shannon; Lee, Sandra L.; Chao, Hsu; Dinh, Huyen; Han, Yi; Doddapaneni, Harshavardhan; Worley, Kim C.; Muzny, Donna M.; Wheeler, David; Panfilio, Kristen A.; Jentzsch, Iris M. Vargas; Vargo, Edward L.; Booth, Warren; Friedrich, Markus; Weirauch, Matthew T.; Anderson, Michelle A. E.; Jones, Jeffery W.; Mittapalli, Omprakash; Zhao, Chaoyang; Zhou, Jing-Jiang; Evans, Jay D.; Attardo, Geoffrey M.; Robertson, Hugh M.; Zdobnov, Evgeny M.; Ribeiro, Jose M. C.; Gibbs, Richard A.; Werren, John H.; Palli, Subba R.; Schal, Coby; Richards, Stephen (Nature, 2016-02-02)
    The bed bug, Cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. This global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. Analyses of the C. lectularius sequenced genome (650Mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory repertoire of genes related to obligate hematophagy, host–symbiont interactions, and several mechanisms of insecticide resistance. In addition, we document the presence of multiple putative lateral gene transfer events. Genome sequencing and annotation establish a solid foundation for future research on mechanisms of insecticide resistance, human–bed bug and symbiont–bed bug associations, and unique features of bed bug biology that contribute to the unprecedented success of C. lectularius as a human ectoparasite