Browsing by Author "Semtner, Paul J."
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- 1976 Virginia insect control guideRoberts, J. E; Robinson, William H.; Weidhass, J. A.; Allen, William A.; Horsburgh, R. L; Semtner, Paul J. (Virginia Cooperative Extension Service, 1976-01)Insect control guide including those in field crops, on animals, fruit, horticulture and vegetable crops, man and household insects, ornamentals, lawn and turf grass, Christmas trees, and nursery and shade trees.
- 1998 Dark-fired Tobacco Production GuideReed, T. David; Jones, J. L.; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 1997)
- 2014 Flue-Cured Tobacco Production GuideReed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2014)An annually-produced guide to the production of flue-cured tobacco, issued in cooperation with the Virginia Bright Flue-Cured Tobacco Board.
- 2015 Flue-Cured Tobacco Production GuideReed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2015)An annually-produced guide to the production of flue-cured tobacco, issued in cooperation with the Virginia Bright Flue-Cured Tobacco Board.
- 2017 Flue-Cured Tobacco Production GuideReed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2017)An annually-produced guide to the production of flue-cured tobacco, issued in cooperation with the Virginia Bright Flue-Cured Tobacco Board.
- Burley Tobacco Production Guide, 2008Peek, Danny R.; Reed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2008)This manual provides information on selecting varieties, greenhouse transplants, and management practices, fertilization, crop rotations, cover crops, sucker control, disease control, weed control, insect pests, curing, and other topics.
- Burley Tobacco Production Guide, 2011Peek, Danny R.; Reed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2011)This report provides data on burley tobacco test plots, including agronomic practices, greenhouse management, fertilizer use, crop rotation, cover crops, sucker control, and control of diseases, weeds and insect pests. Also discusses curing practices.
- Flue-cured Tobacco Production Guide, 2008Reed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2009)This manual provides information on selecting varieties, greenhouse transplants, and management practices, fertilization, crop rotations, cover crops, sucker control, disease control, weed control, insect pests, curing, and other topics.
- Flue-cured Tobacco Production Guide, 2011Reed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2010)Discusses agronomic practices for Flue-cured Tobacco including selection of varieties, greenhouse management, fertilizer use, crop rotation and cover crops, sucker control, and control of diseases, weeds and insect pests. Also discusses curing practices.
- Flue-cured Tobacco Production Guide, 2012Reed, T. David; Johnson, Charles S.; Semtner, Paul J.; Wilkinson, Carol A. (Virginia Cooperative Extension, 2011)Discusses agronomic practices for Flue-cured Tobacco including selection of varieties, greenhouse management, fertilizer use, crop rotation and cover crops, sucker control, and control of diseases, weeds and insect pests. Also discusses curing practices.
- Genetic analysis of resistance to Myzus persicae (Sulzer) in Nicotiana tabacum L.Hinga, Clark D. (Virginia Tech, 1985-08-15)Field experiments with the green peach aphid on tobacco were conducted at Blackstone, Virginia in 1983 and 1984. The objectives were to: 1) confirm and identify source materials resistant to the green peach aphid; 2) study the inheritance of aphid resistance; 3) verify heritability of resistant genotypes through F2 and advanced generation testing and 4) investigate the nature of the resistance. Results showed green peach aphid resistance in Tobacco Introductions 1462, 1118, 1112, 1024, breeding line 1-35 and cultivar NC 745. Inheritance studies showed that the resistance is a recessive trait controlled by three separate, non-linked loci; such that a homozygous recessive at any one locus will condition for the resistance reaction. Among the source materials, one locus conditioned the resistance shown by TI 1118, TI 1112 and breeding line I-35. A second independent locus conditioned the found in TI 1024 and a third independent locus conditioned the resistance observed in NC 745. Tobacco Introduction 1462 possessed alleles for resistance at both the second and third loci. Small aphid cages were of questionable value for studying the resistance reaction. Higher leaf temperatures were noted for the caged leaf surfaces and may be responsible for the unreliable results. Evaluation of F2, F3, F5, F5 populations developed from resistant x susceptible crosses indicated that aphid resistance is a heritable trait and is not closely linked to adverse agronomic quality characteristics.
- Insects and insecticide use on flue-cured tobacco in Virginia during 1979Semtner, Paul J. (Virginia Agricultural Experiment Station, 1982-05)
- Intraclonal Morphological Plasticity within the Myzus persicae (Sulzer) Complex Related to Host Plant and TemperatureMarie, Joan (Virginia Tech, 2004-04-22)Blackman (1987) used life cycle and morphology to separate Myzus nicotianae Blackman, a tobacco-feeding species of aphid, from Myzus persicae (Sulzer). In the present study, the first objective was to investigate the influence of temperature and host plant on the morphology of M. nicotianae and M. persicae. The second objective was to assess Blackman's 1987 key to Myzus for separating tobacco and non-tobacco originating morphs under different environmental conditions. Four host plants were used: tobacco, turnip, pepper, and okra, and three temperatures, 15°C, 20°C, and 25°C. The intraclonal plasticity of two tobacco collected morphs and one turnip collected morph was investigated in relation to these combinations of host and temperature in a 4 x 3 x 3 factorial experimental design. Fifth generation mature apterous aphids were mounted on slides and 10 different morphological structures utilized in morphometric analysis were measured. Data support a morphologically distinct, host-adapted tobacco race but not a separate tobacco-feeding species of M. persicae. The key developed by Blackman (1987) did not discriminate between the tobacco and non-tobacco originating clones but the canonical variates generated from the analysis successfully separated the tobacco and non-tobacco groups. Other studies have used many different clones to investigate the possible distinctions between M. persicae and M. nicotianae; the objective here was to see how much morphological perturbation may be induced within a clone by rearing at different temperatures and on different host plants. Temperature and host plant had substantial influences on the morphology of these aphids. The physiological interactions of temperature-host plant-aphid morphology are very complex yet controlling only for temperature and host plant was sufficient to group specimens according to these independent variables with remarkable accuracy using the linear discriminant functions generated with these data. Percent of aphids in which rearing temperature was correctly identified using linear discriminant functions generated for temperature classes was 87%, 63%, and 64% for 15°C, 20°C, and 25°C, respectively. Random designations would be 33%. Correct identification of host plant was 65%, 45%, 47%, and 48% successful for tobacco, turnip, pepper, and okra, respectively. Random designations for host plant would be 25%. Canonical variates produced clusters by host, temperature, morph, and combinations of these independent variables with varying degrees of discreteness. CV1 by CV2 for host plants gave a very distinct cluster for tobacco and also separate groupings for aphids reared on turnip and pepper. Aphids from the host plant okra were scattered quite widely across the CV1 by CV2 graph. CV1 by CV2 for temperature conditions showed a tight cluster for aphids from 15°C and still distinct though less closely grouped clusters for both 20°C and 25°C rearing temperatures. CV1 by CV2 for the three morphs gave substantial overlap for the two tobacco originating morphs and a more separate cluster for the morph originally collected from turnip.
- Potential of Pandora neoaphidis (Remaudière & Hennebert) Humber as a fungal pathogen for the control of tobacco aphid, Myzus nicotianae Blackman, on tobaccoDara, Surendra Kumar (Virginia Tech, 1995-12-05)The potential of Pandora neoaphidis (Remaudiére & Hennebert) Humber as a fungal pathogen for the control of the tobacco aphid, Myzus nicotianae Blackman, on tobacco was evaluated in a 4-year study between 1992 and 1995. The objectives of this study included determination of the seasonal incidence of P. neoaphidis in populations of tobacco aphid on tobacco and non solanaceous host plants, within plant distribution of the pathogen in aphids on tobacco, influence of tobacco cultivars and cultural practices on the incidence of the pathogen, methods of artificially introducing the pathogen into tobacco aphid populations and their potential in controlling aphids relative to chemical control, virulence of the Virginia isolate of the pathogen to tobacco aphid and green peach aphid, M. persicae (Sulzer), from different geographic locations in the eastern United States, and influence of temperature and type of substrate on the developmental morphology of the pathogen. Infections as high as 91% occurred in aphid populations on tobacco under favorable weather. The pathogen survived at moderate levels in the red morph of tobacco aphid on non solanaceous hosts during fall, parts of winter and spring. P. neoaphidis infections in aphids tended to increase towards the upper leaf positions. Incidence of the pathogen in aphid populations varied widely on various cultivars and types of tobacco. Planting date, topping of tobacco, and stage at which tobacco was topped did not influence the incidence of P. neoaphidis in tobacco aphids. Artificial introduction of P. neoaphidis successfully established infections in tobacco aphids, but failed to prevent the build up of aphid populations. P. neoaphidis was equally virulent to the red and green morphs of the tobacco aphid, and the green peach aphid. The developmental morphology of P. neoaphidis was influenced by temperature and was similar on the surfaces of living substrates, tobacco aphid and tobacco leaf, but different on the inert surface of the coverslip.
- Quantification of insecticide resistance in the tobacco-adapted form of the green peach aphid, Myzus persicae (Sulzer)(Hemiptera: Aphididae)Srigiriraju, Lakshmipathi (Virginia Tech, 2008-04-10)The tobacco-adapted form of the green peach aphid, Myzus persicae (Sulzer), is one of the most important insect pests of tobacco in the United States and around the world. Insecticides play a major role in controlling the aphid on tobacco because natural enemies usually fail to maintain its populations below damaging levels. The aphid has a history of developing resistance to many insecticides. Therefore, baseline information on the aphid's susceptibility to imidacloprid and other insecticides is critical for developing future resistant management programs to minimize losses attributed to the aphid. Studies were conducted on colonies of the tobacco-adapted form of the green peach aphid collected from nine states in the eastern United States in 2004-2007. The susceptibility of 151 colonies to imidacloprid was determined in serial leaf-dip bioassays. When combined over the four years, 18, 14, and 4% of the colonies had 10- to 20-fold, 20- to 30-fold, and 30- to 90-fold resistance ratios, respectively, suggesting that high levels of resistance to imidacloprid are present in field populations of the aphid. A colony collected near Clayton, NC had the highest LC50 value (31 ppm) combined over six tests and three years, but the average resistance ratios for the first three runs was over 130-fold (48 ppm). Geographic location had little effect on susceptibility to imidacloprid. Aphid colonies (136) including red, green, and orange color morphs were screened for total esterase activity using microplate assay with 1-Naphthyl acetate as the substrate. The green morphs generally had lower esterase levels than the red and orange morphs. All orange morphs had among the highest esterase activities. Esterase activities of red and green morphs were positively correlated with LC50 values as determined by leaf-dip bioassays for acephate and methomyl. All 25 colonies tested for esterase gene amplification had either E4 or FE4 gene amplification. The amplification of both E4 and FE4 seen as an 865-bp band characteristic of the FE4 gene and an additional 381-bp band characteristic of a deleted upstream region of the E4 gene occurred in all (4) orange morphs and one (1 of 9) green morph. Target-site insensitivity of acetylcholinesterase (AChE), as modified AChE resistance (MACE) was assessed in 65 colonies of field-collected tobacco-adapted forms of M. persicae. Eight colonies over a range of AChE activity were selected to study inhibition of AChE in the presence of two carbamate insecticides, methomyl and pirimicarb. IC50 values for methomyl ranged from 0.35 to 2.4 μM while six of eight colonies had lower values with a range of 0.16 to 0.30 μM for pirimicarb. Two colonies that were inhibited by methomyl had very high IC50 values of 40.4 and 98.6 μM for pirimicarb. Such insensitivity may be due to mutations in the ace2 gene, but this needs to be confirmed by genetic and molecular analysis. Glutathione S- transferases (GSTs), isoenzymes that are involved in the metabolism and detoxification of many xenobiotic compounds were quantified for 100 colonies by CDNB conjugation. There was a wide range of GST activity for the red (8 to 343 pmol/min/mg protein) and green (15.3 to 330 pmol/min mg protein) morphs, but all six orange morphs collected in 2007 had a narrower range (160 to 211 pmol/min/mg protein). About 45% of the red morphs had GST activity from 200-300 pmol/min/mg of protein, while 53% of the green morphs had a range of 100-200 pmol/min/mg protein. The influence of temperature-mediated synergisms on the toxicity of insecticides in red and green color morphs of the tobacco-adapted from of M. persicae were evaluated using leaf-dip bioassay procedures in laboratory incubators. Post-exposure temperatures of 15, 20, and 25°C were evaluated for four classes of insecticides, acephate, imidacloprid, lambda-cyhalothrin, and methomyl. The temperature change from 15 to 20°C caused almost a 3-fold increase in toxicity to the red and green color morphs for methomyl, acephate, and imidacloprid. In contrast, the toxicity of lambda-cyhalothrin decreased as the temperature increased, showing a negative temperature coefficient. Bioassay experiments conducted with the red morph for indirect estimates of imidacloprid concentrations in flue-cured tobacco showed that leaf position, imidacloprid rate and time after application affected the concentration of the toxicant in the leaf. The differences in aphid mortality between the lower and upper leaf positions indicate that the concentration of imidacloprid and its metabolites were unevenly distributed with the lowest mortality for aphids feeding on the younger, upper leaves and the highest for those feeding on the older, lower leaves. In field experiments, higher aphid populations occurred on tobacco treated with imidacloprid less than the field recommended rate of 41.4 ml/1,000 plants. The development of aphid populations in the field was consistent with the laboratory bioassays. Field trials were conducted to evaluate the performance of various insecticides currently registered for aphid control on tobacco. Imidacloprid applied as a tray drench treatment and acephate as foliar sprays were the most effective treatments. Moderate declines in control with imidacloprid were observed at 75-87 d after transplanting in 2006 and 2007. Aldicarb gave good to excellent control in one of three years, but only fair to poor control in the other two years. Methomyl and lambda-cyhalothrin gave good control in all three years except the residual was shorter. The poor performance of aldicarb in the two years may have been related to the presence of E4 or FE4 resistance in the naturally occurring TGPA in the experimental plots.
- The relationship of time of year, geographic location, insecticide exposure and the genotype of red and green morphs of the tobacco aphid, myzus nicotianae Blackman, in VirginiaBarnes, Martha Letcher (Virginia Tech, 1990-05-07)The relationship of geographic location, insecticide exposure, time of season and the genotype of red and green morphs of the tobacco aphid, Myzus nicotianae Blackman, on tobacco was investigated in Virginia in 1988 and 1989. Color morph and karyotype were found to be highly related. The translocated karyotype was associated with the red morphs and the normal karyotype was associated with the green morphs. The karyotype of red and green morphs did not change markedly from the beginning to the end of the tobacco growing season. Studies were also conducted to determine if the red and green morphs of the tobacco aphid were developing resistance to acephate (Orthene Tobacco Insect Spray), the most commonly used aphicide on flue-cured tobacco in Virginia. Slight resistance was present in both red and green aphid populations from several counties. Studies were also conducted to determine if males were present in tobacco aphid populations in Virginia. Cool temperatures and short photoperiods which initiate male production in Myzus persicae (Sulzer) with holocyclic life cycles did not cause the production of males in M. nicotianae native to Virginia.