Browsing by Author "Takacs-Vesbach, Cristina D."
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- Environmental controls over bacterial communities in polar desert soilsGeyer, Kevin M.; Altrichter, Adam E.; Van Horn, David J.; Takacs-Vesbach, Cristina D.; Gooseff, Michael N.; Barrett, John E. (Ecological Society of America, 2013-10)Productivity-diversity theory has proven informative to many investigations seeking to understand drivers of spatial patterns in biotic communities and relationships between resource availability and community structure documented for a wide variety of taxa. For soil bacteria, availability of organic matter is one such resource known to influence diversity and community structure. Here we describe the influence of environmental gradients on soil bacterial communities of the McMurdo Dry Valleys, Antarctica, a model ecosystem that hosts simple, microbially-dominated foodwebs believed to be primarily structured by abiotic drivers such as water, organic matter, pH, and electrical conductivity. We sampled 48 locations exhibiting orders of magnitude ranges in primary production and soil geochemistry (pH and electrical conductivity) over local and regional scales. Our findings show that environmental gradients imposed by cryptogam productivity and regional variation in geochemistry influence the diversity and structure of soil bacterial communities. Responses of soil bacterial richness to carbon content illustrate a productivity-diversity relationship, while bacterial community structure primarily responds to soil pH and electrical conductivity. This diversity response to resource availability and a community structure response to environmental severity suggests a need for careful consideration of how microbial communities and associated functions may respond to shifting environmental conditions resulting from human activity and climate variability.
- Factors Controlling Soil Microbial Biomass and Bacterial Diversity and Community Composition in a Cold Desert Ecosystem: Role of Geographic ScaleVan Horn, David J.; Van Horn, M. Lee; Barrett, John E.; Gooseff, Michael N.; Altrichter, Adam E.; Geyer, Kevin M.; Zeglin, Lydia H.; Takacs-Vesbach, Cristina D. (PLOS, 2013-06-18)Understanding controls over the distribution of soil bacteria is a fundamental step toward describing soil ecosystems, understanding their functional capabilities, and predicting their responses to environmental change. This study investigated the controls on the biomass, species richness, and community structure and composition of soil bacterial communities in the McMurdo Dry Valleys, Antarctica, at local and regional scales. The goals of the study were to describe the relationships between abiotic characteristics and soil bacteria in this unique, microbially dominated environment, and to test the scale dependence of these relationships in a low complexity ecosystem. Samples were collected from dry mineral soils associated with snow patches, which are a significant source of water in this desert environment, at six sites located in the major basins of the Taylor and Wright Valleys. Samples were analyzed for a suite of characteristics including soil moisture, pH, electrical conductivity, soil organic matter, major nutrients and ions, microbial biomass, 16 S rRNA gene richness, and bacterial community structure and composition. Snow patches created local biogeochemical gradients while inter-basin comparisons encompassed landscape scale gradients enabling comparisons of microbial controls at two distinct spatial scales. At the organic carbon rich, mesic, low elevation sites Acidobacteria and Actinobacteria were prevalent, while Firmicutes and Proteobacteria were dominant at the high elevation, low moisture and biomass sites. Microbial parameters were significantly related with soil water content and edaphic characteristics including soil pH, organic matter, and sulfate. However, the magnitude and even the direction of these relationships varied across basins and the application of mixed effects models revealed evidence of significant contextual effects at local and regional scales. The results highlight the importance of the geographic scale of sampling when determining the controls on soil microbial community characteristics.
- Microbial Community Responses to Increased Water and Organic Matter in the Arid Soils of the McMurdo Dry Valleys, AntarcticaBuelow, Heather N.; Winter, Ara S.; Van Horn, David J.; Barrett, John E.; Gooseff, Michael N.; Schwartz, Egbert; Takacs-Vesbach, Cristina D. (Frontiers, 2016-07-18)The soils of the McMurdo Dry Valleys, Antarctica are an extreme polar desert, inhabited exclusively by microscopic taxa. This region is on the threshold of anticipated climate change, with glacial melt, permafrost thaw, and the melting of massive buried ice increasing liquid water availability and mobilizing soil nutrients. Experimental water and organic matter (OM) amendments were applied to investigate how these climate change effects may impact the soil communities. To identify active taxa and their functions, total community RNA transcripts were sequenced and annotated, and amended soils were compared with unamended control soils using differential abundance and expression analyses. Overall, taxonomic diversity declined with amendments of water and OM. The domain Bacteria increased with both amendments while Eukaryota declined from 38% of all taxa in control soils to 8 and 11% in water and OM amended soils, respectively. Among bacterial phyla, Actinobacteria (59%) dominated water-amended soils and Firmicutes (45%) dominated OM amended soils. Three bacterial phyla (Actinobacteria, Proteobacteria, and Firmicutes) were primarily responsible for the observed positive functional responses, while eukaryotic taxa experienced the majority (27 of 34) of significant transcript losses. These results indicated that as climate changes in this region, a replacement of endemic taxa adapted to dry, oligotrophic conditions by generalist, copiotrophic taxa is likely.
- Primary productivity as a control over soil microbial diversity along environmental gradients in a polar desert ecosystemGeyer, Kevin M.; Takacs-Vesbach, Cristina D.; Gooseff, Michael N.; Barrett, John E. (PeerJ, 2017-07-25)Primary production is the fundamental source of energy to foodwebs and ecosystems, and is thus an important constraint on soil communities. This coupling is particularly evident in polar terrestrial ecosystems where biological diversity and activity is tightly constrained by edaphic gradients of productivity (e.g., soil moisture, organic carbon availability) and geochemical severity (e.g., pH, electrical conductivity). In the McMurdo Dry Valleys of Antarctica, environmental gradients determine numerous properties of soil communities and yet relatively few estimates of gross or net primary productivity (GPP, NPP) exist for this region. Here we describe a survey utilizing pulse amplitude modulation (PAM) fluorometry to estimate rates of GPP across a broad environmental gradient along with belowground microbial diversity and decomposition. PAM estimates of GPP ranged from an average of 0.27 μmol O2/m2/s in the most arid soils to an average of 6.97 μmol O2/m2/s in the most productive soils, the latter equivalent to 217 g C/m2/y in annual NPP assuming a 60 day growing season. A diversity index of four carbon-acquiring enzyme activities also increased with soil productivity, suggesting that the diversity of organic substrates in mesic environments may be an additional driver of microbial diversity. Overall, soil productivity was a stronger predictor of microbial diversity and enzymatic activity than any estimate of geochemical severity. These results highlight the fundamental role of environmental gradients to control community diversity and the dynamics of ecosystem-scale carbon pools in arid systems.
- Seasonal controls on snow distribution and aerial ablation at the snow-patch and landscape scales, McMurdo Dry Valleys, AntarcticaEveland, J. W.; Gooseff, Michael N.; Lampkin, D. J.; Barrett, John E.; Takacs-Vesbach, Cristina D. (Copernicus Publications, 2013)Accumulated snow in the McMurdo Dry Valleys, while limited, has great ecological significance to subnivian soil environments. Though sublimation dominates the ablation process in this region, measurable increases in soil moisture and insulation from temperature extremes provide more favorable conditions with respect to subnivian soil communities. While precipitation is not substantial, significant amounts of snow can accumulate, via wind transport, in topographic lees along the valley bottoms, forming thousands of discontinuous snow patches. These patches have the potential to act as significant sources of local meltwater, controlling biogeochemical cycling and the landscape distribution of microbial communities. Therefore, determining the spatial and temporal dynamics of snow at multiple scales is imperative to understanding the broader ecological role of snow in this region. High-resolution satellite imagery acquired during the 2009-2010 and 2010-2011 austral summers was used to quantify the distribution of snow across Taylor and Wright valleys. Extracted snow-covered area from the imagery was used as the basis for assessing inter-annual variability and seasonal controls on accumulation and ablation of snow at multiple scales. In addition to landscape analyses, fifteen 1 km(2) plots (3 in each of 5 study regions) were selected to assess the prevalence of snow cover at finer spatial scales, referred to herein as the snow-patch scale. Results confirm that snow patches tend to form in the same locations each year with some minor deviations observed. At the snow-patch scale, neighboring patches often exhibit considerable differences in aerial ablation rates, and particular snow patches do not reflect trends for snow-covered area observed at the landscape scale. These differences are presumably related to microtopographic influences acting on individual snow patches, such as wind sheltering and differences in snow depth due to the underlying topography. This highlights the importance of both the landscape and snow-patch scales in assessing the effects of snow cover on biogeochemical cycling and microbial communities.
- Spatial variation in soil active-layer geochemistry across hydrologic margins in polar desert ecosystemsBarrett, John E.; Gooseff, Michael N.; Takacs-Vesbach, Cristina D. (Copernicus Publications, 2009)Polar deserts are characterized by severe spatial-temporal limitations of liquid water. In soil active layers of the Antarctic Dry Valleys, liquid water is infrequently available over most of the arid terrestrial landscape. However, soils on the margins of glacial melt-water streams and lakes are visibly wet during the brief Austral summer when temperatures permit the existence of liquid water. We examined the role of these hydrologic margins as preferential zones for the transformation and transport of nutrient elements and solutes in an environment where geochemical weathering and biological activity is strictly limited by the dearth of liquid water. We report on hydropedological investigations of aquatic-terrestrial transition zones adjacent to 11 stream and lake systems in the Antarctic Dry Valleys. Our results show that wetted zones extended 1-11 m from the edges of lotic and lentic systems. While capillary demand and surface evaporation drive a one-way flux of water through these zones, the scale of these transition zones is determined by the topography and physical characteristics of the surrounding soils. Nutrient concentrations and fluxes appear to be influenced by both the hydrology and microbial-mediated biogeochemical processes. Salt concentrations are enriched near the distal boundary of the wetted fronts due to evapo-concentration of pore water in lake margin soils, while organic matter, ammonium and phosphate concentrations are highest in stream channel sediments where potential for biological activity is greatest. Thus, in the Antarctic Dry Valleys, intermittently wet soils on the margins of streams and lakes are important zones of both geochemical cycling and biological activity.