Browsing by Author "Umaña, Ariana"
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- Complete Genome Sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286Umaña, Ariana; Lemkul, Justin A.; Slade, Daniel J. (American Society for Microbiology, 2019-02-21)Fusobacterium necrophorum is a pathogenic Gram-negative, anaerobic bacterium. In this study, we present the first complete genome sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286. These data provide a critical advancement in our understanding of virulence factors that could contribute to F. necrophorum pathogenesis in both human and livestock infections.
- FusoPortal: an Interactive Repository of Hybrid MinIONSequenced Fusobacterium Genomes Improves Gene Identification and CharacterizationSanders, Blake E.; Umaña, Ariana; Lemkul, Justin A.; Slade, Daniel J. (American Society for Microbiology, 2018-07-05)Here we present FusoPortal, an interactive repository of Fusobacterium genomes that were sequenced using a hybrid MinION long-read sequencing pipeline, followed by assembly and annotation using a diverse portfolio of predominantly open-source software. Significant efforts were made to provide genomic and bioinformatic data as downloadable files, including raw sequencing reads, genome maps, gene annotations, protein functional analysis and classifications, and a custom BLAST server for FusoPortal genomes. FusoPortal has been initiated with eight complete genomes, of which seven were previously only drafts that ranged from 24 to 67 contigs. We have showcased that the genomes in FusoPortal provide accurate open reading frame annotations and have corrected a number of large (3-kb) genes that were previously misannotated due to contig boundaries. In summary, FusoPortal (http://fusoportal.org) is the first database of MinION-sequenced and completely assembled Fusobacterium genomes, and this central Fusobacterium genomic and bioinformatic resource will aid the scientific community in developing a deeper understanding of how this human pathogen contributes to an array of diseases, including periodontitis and colorectal cancer.