Browsing by Author "Wang, Yunmiao"
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- Microgap Structured Optical Sensor for Fast Label-free DNA DetectionWang, Yunmiao (Virginia Tech, 2011-05-03)DNA detection technology has developed rapidly due to its extensive application in clinical diagnostics, bioengineering, environmental monitoring, and food science areas. Currently developed methods such as surface Plasmon resonance (SPR) methods, fluorescent dye labeled methods and electrochemical methods, usually have the problems of bulky size, high equipment cost and time-consuming algorithms, so limiting their application for in vivo detection. In this work, an intrinsic Fabry-Perot interferometric (IFPI) based DNA sensor is presented with the intrinsic advantages of small size, low cost and corrosion-tolerance. This sensor has experimentally demonstrated its high sensitivity and selectivity. In theory, DNA detection is realized by interrogating the sensor's optical cavity length variation resulting from hybridization event. First, a microgap structure based IFPI sensor is fabricated with simple etching and splicing technology. Subsequently, considering the sugar phosphate backbone of DNA, layer-by-layer electrostatic self-assembly technique is adopted to attach the single strand capture DNA to the sensor endface. When the target DNA strand binds to the single-stranded DNA successfully, the optical cavity length of sensor will be increased. Finally, by demodulating the sensor spectrum, DNA hybridization event can be judged qualitatively. This sensor can realize DNA detection without attached label, which save the experiment expense and time. Also the hybridization detection is finished within a few minutes. This quick response feature makes it more attractive in diagnose application. Since the sensitivity and specificity are the most widely used statistics to describe a diagnostic test, so these characteristics are used to evaluate this biosensor. Experimental results demonstrate that this sensor has a sensitivity of 6nmol/ml and can identify a 2 bp mismatch. Since this sensor is optical fiber based, it has robust structure and small size ( 125μm ). If extra etching process is applied to the sensor, the size can be further reduced. This promises the sensor potential application of in-cell detection. Further investigation can be focused on the nanofabrication of this DNA sensor, and this is very meaningful topic not only for diagnostic test but also in many other applications such as food industry, environment monitoring.
- Photonic Biosensor Assays to Detect and Distinguish Subspecies of Francisella tularensisCooper, Kristie L.; Bandara, Aloka B.; Wang, Yunmiao; Wang, Anbo; Inzana, Thomas J. (MDPI, 2011-03-07)The application of photonic biosensor assays to diagnose the category-A select agent Francisella tularensis was investigated. Both interferometric and long period fiber grating sensing structures were successfully demonstrated; both these sensors are capable of detecting the optical changes induced by either immunological binding or DNA hybridization. Detection was made possible by the attachment of DNA probes or immunoglobulins (IgG) directly to the fiber surface via layer-by-layer electrostatic self-assembly. An optical fiber biosensor was tested using a standard transmission mode long period fiber grating of length 15 mm and period 260 µm, and coated with the IgG fraction of antiserum to F. tularensis. The IgG was deposited onto the optical fiber surface in a nanostructured film, and the resulting refractive index change was measured using spectroscopic ellipsometry. The presence of F. tularensis was detected from the decrease of peak wavelength caused by binding of specific antigen. Detection and differentiation of F. tularensis subspecies tularensis (type A strain TI0902) and subspecies holarctica (type B strain LVS) was further accomplished using a single-mode multi-cavity fiber Fabry-Perot interferometric sensor. These sensors were prepared by depositing seven polymer bilayers onto the fiber tip followed by attaching one of two DNA probes: (a) a 101-bp probe from the yhhW gene unique to type-A strains, or (b) a 117-bp probe of the lpnA gene, common to both type-A and type-B strains. The yhhW probe was reactive with the type-A, but not the type-B strain. Probe lpnA was reactive with both type-A and type-B strains. Nanogram quantities of the target DNA could be detected, highlighting the sensitivity of this method for DNA detection without the use of PCR. The DNA probe reacted with 100% homologous target DNA, but did not react with sequences containing 2-bp mismatches, indicating the high specificity of the assay. These assays will fill an important void that exists for rapid, culture-free, and field-compatible diagnosis of F. tularensis.
- A Quasi-distributed Sensing Network Based on Wavelength-Scanning Time-division Multiplexed Fiber Bragg GratingsWang, Yunmiao (Virginia Tech, 2012-08-28)Structural health monitoring (SHM) has become a strong national interest because of the need of reliable and accurate damage detection methods for aerospace, civil and mechanical engineering infrastructure. Health monitoring of these structures usually requires the sensors to have such features as large area coverage, maintenance free or minimum maintenance, ultra-low cost per measurement point, and capability of operation in harsh environments. Fiber Bragg grating (FBG) has attracted considerable interest for this application because of its compactness, electromagnetic immunity, and excellent multiplexing capability. Several FBG multiplexing techniques have been developed to increase the multiplexing number and further reduce the unit cost. To the author's best knowledge, the current demonstrated maximum multiplexing number are 800 FBG sensors in a single array using optical frequency domain reflectometry (OFDR), whose maximum fiber span is limited by the coherence length of light source. In this work, we proposed and demonstrated a wavelength-scanning time-division multiplexing (WSTDM) of 1000 ultra-weak FBGs for distributed temperature sensing. In comparison with the OFDR method, the WSTDM method distinguishes the sensors by different time delays, and its maximum operation distance, which is limited by the transmission loss of the fiber, can be as high as tens of kilometers. The strong multiplexing capability and low crosstalk of the ultra-weak FBG sensors was investigated through both theoretical analysis and experiment. An automated FBG fabrication system was developed for fast FBG fabrication. With this WSTDM method, we multiplexed 1000 ultra-weak FBGs for distributed temperature sensing. Besides the demonstrated temperature measurement, the reported method can also be applied to measure other parameters, such as strain, pressure.