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Browsing by Author "Wilson, William"

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    Kant on the Progression of Representation
    Wilson, William (Virginia Tech, 2017-06-03)
    Recently, the key point of contention in Kant scholarship has revolved around a question concerning whether, for Kant, intuitions can play their role of presenting objects to the mind without the discursive activity of the intellect. According to 'conceptualist' interpretations, intuitions depend for their generation on the activity of the understanding. According to 'nonconceptualist' interpretations, at least some intuitions do not depend for their generation on the activity of the understanding. I argue that although the conceptualism/nonconceptualism debate has brought greater clarity to a number of issues within Kant's critical philosophy, the debate partially rests on a conflation of two importantly distinct representational states, namely 'intuition' [Anschaaung] and 'perception' [Wahrnehmung]. I argue that once this distinction is noted, many of the passages that would appear to threaten a nonconceptualist interpretation lose their force. In addition, I argue that if we understand the conceptualist claim in terms of the kind of structure a particular representational state possesses, then we have good reason to reject the idea that, for Kant, sensory experience is fundamentally conceptual in character.
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    Rift Valley fever virus Gn V5-epitope tagged virus enables identification of UBR4 as a Gn interacting protein that facilitates Rift Valley fever virus production
    Bracci, Nicole; de la Fuente, Cynthia; Saleem, Sahar; Pinkham, Chelsea; Narayanan, Aarthi; Garcia-Sastre, Adolfo; Balaraman, Velmurugan; Richt, Juergen A.; Wilson, William; Kehn-Hall, Kylene (Academic Press, 2022-02)
    Rift Valley fever virus (RVFV) is an arbovirus that was first reported in the Rift Valley of Kenya which causes significant disease in humans and livestock. RVFV is a tri-segmented, negative-sense RNA virus consisting of a L, M, and S segments with the M segment encoding the glycoproteins Gn and Gc. Host factors that interact with Gn are largely unknown. To this end, two viruses containing an epitope tag (V5) on the Gn protein in position 105 or 229 (V5Gn105 and V5Gn229) were generated using the RVFV MP-12 vaccine strain as a backbone. The V5-tag insertion minimally impacted Gn functionality as measured by replication kinetics, Gn localization, and antibody neutralization assays. A proteomics-based approach was used to identify novel Gn-binding host proteins, including the E3 ubiquitin-protein ligase, UBR4. Depletion of UBR4 resulted in a significant decrease in RVFV titers and a reduction in viral RNA production.