Browsing by Author "Yang, Anna"

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    Vacuum Steam Treatment Eradicates Viable Bretziella fagacearum from Logs Cut from Wilted Quercus rubra
    Juzwik, Jennifer; Yang, Anna; Chen, Zhangjing; White, Marshall S.; Shugrue, Sarah; Mack, Ronald (2019-02)
    Methyl bromide (MB) fumigation is required for U.S. Quercus log exports due to concern over unintentional spread of Bretziella fagacearum. MB alternatives are needed due to the chemical's ability to damage the earth's ozone layer. Vacuum steam (VS) is an environmentally friendly method that was evaluated for its ability to eradicate B. fagacearum in logs (24 to 61 cm diameter; 1.7 to 1.9 m long) obtained from Q. rubra that had wilted following natural infection (NI) or artificial inoculation (Al). Five replicate loads of two NI and one AI logs were VS-treated at 56 degrees C for 30 min and 60 degrees C for 60 min (at 5.0 cm sapwood depth). Mean frequencies of pretreatment fungus colonization ranged from 12.5 to 24.4% in NI and 29.4 to 45.6% in AI logs based on isolation from wood chips of inner and outer sapwood, respectively, of two disks per log. Frequencies of pathogen DNA detection were similar to those for isolation. No viable pathogen or its DNA were detected in posttreatment logs. Treatment times ranged from 5 to 9 h for the 56 degrees C/30 min schedule and from 8 to 10 h for the 60 degrees C/60 min schedule. Based on these results, VS is worthy of further development as a MB alternative.
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    Vacuum Steam Treatment of Metrosideros polymorpha Logs for Eradication of Ceratocystis huliohia and C. lukuohia
    Juzwik, Jennifer; Hughes, Marc A.; Chen, Zhangjing; Yang, Anna; Keith, Lisa M.; White, Marshall S. (American Phytopathological Society, 2022-04)
    A new and devastating disease, rapid ohia death (ROD), in Hawaii led to a state quarantine that regulates interisland transport of ohia wood and plant material to prevent spread of the causal pathogens. Heat treatments of ohia logs in commercial trade were considered for phytosanitary treatment. Vacuum steam (VS) was evaluated for its ability to eradicate the pathogens, Ceratocystis lukuohia and C. huliohia, in main stem logs from ROD-affected forest trees. Replicate loads of three debarked logs (24 to 43 cm in diameter, 1.7 to 2.0 m long) were VS treated at 56 degrees C for 30 min (five loads) or 60 degrees C for 60 min (four loads) at a sapwood depth equal to 70% of log radius. Percentage isolation of Ceratocystis from VS and ambient temperature logs before treatment and summarized by source tree ranged from 12 to 66% and 6 to 31% based on carrot baiting assays of tissue taken from outer and inner sapwood, respectively. No viable Ceratocystis was detected in sapwood locations for the 60 degrees C/60 min schedule or inner locations for the 56 degrees C/30 min schedule after treatment. Only one subsample (0.48%, n = 208) of the latter schedule treatment yielded Ceratocystis. Time needed for treatment ranged from 7.4 to 15 h for the 56 degrees C/30 min schedule and from 8.6 to 19.2 h for the 60 degrees C/60 min schedule. These results demonstrate that VS is an effective and efficient method for treating large-diameter ohia logs that mill owners and regulatory plant pathologists may consider for use in Hawaii.