Browsing by Author "Yang, Xiaohan"

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    Effects of Hydraulic Retention Time and Influent Nitrate-N Concentration on Nitrogen Removal and the Microbial Community of an Aerobic Denitrification Reactor Treating Recirculating Marine Aquaculture System Effluent
    Song, Xiefa; Yang, Xiaohan; Hallerman, Eric M.; Jiang, Yuli; Huang, Zhitao (MDPI, 2020-02-28)
    The effects of hydraulic retention time (HRT) and influent nitrate-N concentration on nitrogen removal and the microbial community composition of an aerobic denitrification reactor treating recirculating marine aquaculture system effluent were evaluated. Results showed that over 98% of nitrogen was removed and ammonia-N and nitrite-N levels were below 1 mg/L when influent nitrate-N was below 150 mg/L and HRT over 5 h. The maximum nitrogen removal efficiency and nitrogen removal rate were observed at HRT of 6 or 7 h when influent nitrate-N was 150 mg/L. High-throughput DNA sequencing analysis revealed that the microbial phyla Proteobacteria and Bacteroidetes were predominant in the reactor, with an average relative total abundance above 70%. The relative abundance of denitrifying bacteria of genera Halomonas and Denitratisoma within the reactor decreased with increasing influent nitrate-N concentrations. Our results show the presence of an aerobically denitrifying microbial consortium with both expected and unexpected members, many of them relatively new to science. Our findings provide insights into the biological workings and inform the design and operation of denitrifying reactors for marine aquaculture systems.
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    JGI Plant Gene Atlas: an updateable transcriptome resource to improve functional gene descriptions across the plant kingdom
    Sreedasyam, Avinash; Plott, Christopher; Hossain, Md Shakhawat; Lovell, John T.; Grimwood, Jane; Jenkins, Jerry W.; Daum, Christopher; Barry, Kerrie; Carlson, Joseph; Shu, Shengqiang; Phillips, Jeremy; Amirebrahimi, Mojgan; Zane, Matthew; Wang, Mei; Goodstein, David; Haas, Fabian B.; Hiss, Manuel; Perroud, Pierre-Francois; Jawdy, Sara S.; Yang, Yongil; Hu, Rongbin; Johnson, Jenifer; Kropat, Janette; Gallaher, Sean D.; Lipzen, Anna; Shakirov, Eugene; Weng, Xiaoyu; Torres-Jerez, Ivone; Weers, Brock; Conde, Daniel; Pappas, Marilia R.; Liu, Lifeng; Muchlinski, Andrew; Jiang, Hui; Shyu, Christine; Huang, Pu; Sebastian, Jose; Laiben, Carol; Medlin, Alyssa; Carey, Sankalpi; Carrell, Alyssa A.; Chen, Jin-Gui; Perales, Mariano; Swaminathan, Kankshita; Allona, Isabel; Grattapaglia, Dario; Cooper, Elizabeth A.; Tholl, Dorothea; Vogel, John P.; Weston, David J.; Yang, Xiaohan; Brutnell, Thomas P.; Kellogg, Elizabeth A.; Baxter, Ivan; Udvardi, Michael; Tang, Yuhong; Mockler, Todd C.; Juenger, Thomas E.; Mullet, John; Rensing, Stefan A.; Tuskan, Gerald A.; Merchant, Sabeeha S.; Stacey, Gary; Schmutz, Jeremy (Oxford University Press, 2023-08-01)
    Gene functional descriptions offer a crucial line of evidence for candidate genes underlying trait variation. Conversely, plant responses to environmental cues represent important resources to decipher gene function and subsequently provide molecular targets for plant improvement through gene editing. However, biological roles of large proportions of genes across the plant phylogeny are poorly annotated. Here we describe the Joint Genome Institute (JGI) Plant Gene Atlas, an updateable data resource consisting of transcript abundance assays spanning 18 diverse species. To integrate across these diverse genotypes, we analyzed expression profiles, built gene clusters that exhibited tissue/condition specific expression, and tested for transcriptional response to environmental queues. We discovered extensive phylogenetically constrained and condition-specific expression profiles for genes without any previously documented functional annotation. Such conserved expression patterns and tightly co-expressed gene clusters let us assign expression derived additional biological information to 64 495 genes with otherwise unknown functions. The ever-expanding Gene Atlas resource is available at JGI Plant Gene Atlas (https://plantgeneatlas.jgi.doe.gov) and Phytozome (https://phytozome.jgi.doe.gov/), providing bulk access to data and user-specified queries of gene sets. Combined, these web interfaces let users access differentially expressed genes, track orthologs across the Gene Atlas plants, graphically represent co-expressed genes, and visualize gene ontology and pathway enrichments.