Browsing by Author "Zahid, Osama K."
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- Label-free analysis of physiological hyaluronan size distribution with a solid-state nanopore sensorRivas, Felipe; Zahid, Osama K.; Reesink, Heidi L.; Peal, Bridgette T.; Nixon, Alan J.; DeAngelis, Paul L.; Skardal, Aleksander; Rahbar, Elaheh; Hall, Adam R. (Springer Nature, 2018-03-12)Hyaluronan (or hyaluronic acid, HA) is a ubiquitous molecule that plays critical roles in numerous physiological functions in vivo, including tissue hydration, inflammation, and joint lubrication. Both the abundance and size distribution of HA in biological fluids are recognized as robust indicators of various pathologies and disease progressions. However, such analyses remain challenging because conventional methods are not sufficiently sensitive, have limited dynamic range, and/or are only semi-quantitative. Here we demonstrate label-free detection and molecular weight discrimination of HA with a solid-state nanopore sensor. We first employ synthetic HA polymers to validate the measurement approach and then use the platform to determine the size distribution of as little as 10 ng of HA extracted directly from synovial fluid in an equine model of osteoarthritis. Our results establish a quantitative method for assessment of a significant molecular biomarker that bridges a gap in the current state of the art.
- Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanoporesZahid, Osama K.; Zhao, Boxuan Simen; He, Chuan; Hall, Adam R. (Springer Nature, 2016-07-07)5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA.